XANES spectroscopy sensitivity to small electronic changes Case of carp azidomethemoglobin
Résumé
Spin states equilibrium of hemoglobin‐iron varies with external conditions: pH, allosteric effectors, temperature. The small electronic reorganization of the iron caused by the spin state changes has been detected by X‐ray absorption near edge structure (XANES) spectroscopy at room temperature. The iron K‐edge region which is sensitive to spin state is located in 7110–7130 eV. Here are presented the 100% high spin and 100% low spin XANES spectra of carp azido ferric hemoglobin.