A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs - Archive ouverte HAL
Journal Articles Nucleic Acids Research Year : 1977

A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs

Abstract

A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) are added to valyl-tRNA synthetase, a complex consisting of two enzyme molecules and one tRNA molecule is first formed. It is subsequently dissociated to a one to one complex when more tRNA is present in the solution. The association curve shows a maximum for a molecular ratio, enzyme over tRNA, equal to 2.

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hal-04587876 , version 1 (24-05-2024)

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Richard Giegé, B. Jacrot, Dino Moras, J. C. Thierry, G. Zaccai. A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs. Nucleic Acids Research, 1977, 4 (7), pp.2421-2428. ⟨10.1093/nar/4.7.2421⟩. ⟨hal-04587876⟩
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