<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04581563</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T07:58:04+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Cells need safety valves</title>
            <author role="crp">
              <persName>
                <forename type="first">Antoine</forename>
                <surname>Danchin</surname>
              </persName>
              <email type="md5">87fcb798b65c511bd1947be399e04d0b</email>
              <email type="domain">normalesup.org</email>
              <idno type="idhal" notation="string">adanchin</idno>
              <idno type="idhal" notation="numeric">21434</idno>
              <idno type="halauthorid" notation="string">3919-21434</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-6350-5001</idno>
              <idno type="IDREF">https://www.idref.fr/026810441</idno>
              <affiliation ref="#struct-3440"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Ibrahima</forename>
                <surname>SOUANE</surname>
              </persName>
              <email type="md5">1d28dd422a7d12c25829c5bc1bbd4910</email>
              <email type="domain">cirad.fr</email>
            </editor>
            <funder>This work was supported by the PROBACTYS programme, grant CT-2006-029104, and the TARPOL programme, grant KBBE-2007-212894, in an effort to define genes essential for the construction of a synthetic cell.</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2024-05-21 13:50:21</date>
              <date type="whenModified">2024-06-07 15:09:20</date>
              <date type="whenReleased">2024-05-21 13:50:21</date>
              <date type="whenProduced">2009-06-12</date>
              <ref type="externalLink" target="https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bies.200900024"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="1325623">
                <persName>
                  <forename>Ibrahima</forename>
                  <surname>SOUANE</surname>
                </persName>
                <email type="md5">1d28dd422a7d12c25829c5bc1bbd4910</email>
                <email type="domain">cirad.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04581563</idno>
            <idno type="halUri">https://hal.science/hal-04581563</idno>
            <idno type="halBibtex">danchin:hal-04581563</idno>
            <idno type="halRefHtml">&lt;i&gt;BioEssays&lt;/i&gt;, 2009, 31 (7), pp.769-773. &lt;a target="_blank" href="https://dx.doi.org/10.1002/bies.200900024"&gt;&amp;#x27E8;10.1002/bies.200900024&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">BioEssays, 2009, 31 (7), pp.769-773. &amp;#x27E8;10.1002/bies.200900024&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="PASTEUR">Institut Pasteur</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="FRANCE-GENOMIQUE">France Génomique</idno>
            <idno type="stamp" n="MICROSCOPE" corresp="CEA">Microbial Genome Annotation &amp; Analysis Platform (MAGE)</idno>
          </seriesStmt>
          <notesStmt>
            <note type="commentary">A utilisé MicroScope Platform</note>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Cells need safety valves</title>
                <author role="crp">
                  <persName>
                    <forename type="first">Antoine</forename>
                    <surname>Danchin</surname>
                  </persName>
                  <email type="md5">87fcb798b65c511bd1947be399e04d0b</email>
                  <email type="domain">normalesup.org</email>
                  <idno type="idhal" notation="string">adanchin</idno>
                  <idno type="idhal" notation="numeric">21434</idno>
                  <idno type="halauthorid" notation="string">3919-21434</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-6350-5001</idno>
                  <idno type="IDREF">https://www.idref.fr/026810441</idno>
                  <affiliation ref="#struct-3440"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11144</idno>
                <idno type="issn">0265-9247</idno>
                <idno type="eissn">1521-1878</idno>
                <title level="j">BioEssays</title>
                <imprint>
                  <publisher>Wiley-VCH Verlag</publisher>
                  <biblScope unit="volume">31</biblScope>
                  <biblScope unit="issue">7</biblScope>
                  <biblScope unit="pp">769-773</biblScope>
                  <date type="datePub">2009-06-12</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1002/bies.200900024</idno>
              <idno type="pubmed">19472369</idno>
              <ref type="publisher">https://onlinelibrary.wiley.com/doi/full/10.1002/bies.200900024</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">synthetic biology</term>
                <term xml:lang="en">permease</term>
                <term xml:lang="en">operon</term>
                <term xml:lang="en">genetic addiction</term>
                <term xml:lang="en">bistability</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halDomain" n="sdv.bc">Life Sciences [q-bio]/Cellular Biology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>In Escherichia coli , the role of lacA , the third gene of the lactose operon, has remained an enigma. I suggest that its role is the consequence of the need for cells to have safety valves that protect them from the osmotic effect created by their permeases. Safety valves allow them to cope with the buildup of osmotic pressure under accidental transient conditions. Multidrug resistance (MDR) efflux, thus named because of our anthropocentrism, is ubiquitous. Yet, the formation of simple leaks would result in futile influx/efflux cycles. Versatile modification enzymes with low sensitivity solve the problem if the modified metabolite is the one exported by MDR permeases. This may account for the pervasive presence of acetyltransferases, such as LacA, associated to acetyl‐metabolite exporters. This scenario of constraints imposed by efficient influx of metabolites provides us with a model that should be followed when constructing synthetic cells.</p>
            </abstract>
            <particDesc>
              <org type="consortium">MicroScope Platform</org>
            </particDesc>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-3440" status="OLD">
          <idno type="RNSR">198618099N</idno>
          <orgName>Génétique des Génomes Bactériens</orgName>
          <desc>
            <address>
              <addrLine>28 rue du Docteur Roux 75724 Paris Cedex 15, France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.pasteur.fr/recherche/unites/REG/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-300027" type="direct"/>
            <relation name="URA2171" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300027" status="VALID">
          <idno type="IdRef">027936643</idno>
          <idno type="ISNI">0000 0001 2353 6535</idno>
          <idno type="ROR">https://ror.org/0495fxg12</idno>
          <orgName>Institut Pasteur [Paris]</orgName>
          <orgName type="acronym">IP</orgName>
          <date type="start">1887-06-04</date>
          <desc>
            <address>
              <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.pasteur.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>