A Rhenium(I)-Diselenoether Compound Significantly Decreased the Viral Load of SARS-CoV-2 in Infected Pulmonary Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Transition Metal Complexes Année : 2023

A Rhenium(I)-Diselenoether Compound Significantly Decreased the Viral Load of SARS-CoV-2 in Infected Pulmonary Cells

Philippe Collery
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Sunali Khanna
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Pascal Grondin
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Guillaume Fichet
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Résumé

chymotrypsin-like protease (3CLpro), a cathepsin cysteine protease, which is one of the main proteases of the severe acute respiratory syndrome—coronavirus 2 (SARS-CoV-2 virus), but the antiviral effect was not studied. On the other hand, a rhenium(I)-diselenoether complex (Re-diSe), which is a Re(I)tricarbonyl compound with a diselenide ligand, decreased the production of cysteine proteases B and S in cancer and normal cells at doses that did not affect the viability of the normal cells. Objective. To study the effect of Re-diSe on the viral load of SARS-CoV-2 in infected Calu-3 pulmonary human epithelial cells in culture. Methods. Cells were infected with SARSCoV- 2 (European original origin) with one multiplication of infection (MOI 0.01). The viral load was assayed in the supernatant by RT-qPCR after an exposure time of 120 h in cells treated at different doses (1, 5, 10, and 20 μM) and in non-treated cells (controls). In parallel, viability of the cells was assayed with Cell Titer Glo technique. Results. A significant decrease of the viral load was observed at doses of 10 and 20 μM without any cytotoxity. Discussion. Further studies are required to correlate these effects with the activities of host and virus cysteine proteases, but Re-diSe is already a promising drug for the treatment of Covid19.
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hal-04580013 , version 1 (18-05-2024)

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Philippe Collery, Sunali Khanna, Pascal Grondin, Guillaume Fichet, Didier Desmaële. A Rhenium(I)-Diselenoether Compound Significantly Decreased the Viral Load of SARS-CoV-2 in Infected Pulmonary Cells. Journal of Transition Metal Complexes, 2023, 6, pp.1-5. ⟨10.32371/jtmc/246150⟩. ⟨hal-04580013⟩
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