A Rhenium(I)-Diselenoether Compound Significantly Decreased the Viral Load of SARS-CoV-2 in Infected Pulmonary Cells
Résumé
chymotrypsin-like protease (3CLpro), a cathepsin cysteine protease,
which is one of the main proteases of the severe acute respiratory
syndrome—coronavirus 2 (SARS-CoV-2 virus), but the antiviral
effect was not studied. On the other hand, a rhenium(I)-diselenoether
complex (Re-diSe), which is a Re(I)tricarbonyl compound with a
diselenide ligand, decreased the production of cysteine proteases B
and S in cancer and normal cells at doses that did not affect the
viability of the normal cells. Objective. To study the effect of Re-diSe
on the viral load of SARS-CoV-2 in infected Calu-3 pulmonary human
epithelial cells in culture. Methods. Cells were infected with SARSCoV-
2 (European original origin) with one multiplication of infection
(MOI 0.01). The viral load was assayed in the supernatant by RT-qPCR
after an exposure time of 120 h in cells treated at different doses (1, 5,
10, and 20 μM) and in non-treated cells (controls). In parallel, viability
of the cells was assayed with Cell Titer Glo technique. Results. A
significant decrease of the viral load was observed at doses of 10 and
20 μM without any cytotoxity. Discussion. Further studies are required
to correlate these effects with the activities of host and virus cysteine
proteases, but Re-diSe is already a promising drug for the treatment of
Covid19.
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