Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2012

Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC

Résumé

Background Bacterial genomes displaying a strong bias between the leading and the lagging strand of DNA replication encode two DNA polymerases III, DnaE and PolC, rather than a single one. Replication is a highly unsymmetrical process, and the presence of two polymerases is therefore not unexpected. Using comparative genomics, we explored whether other processes have evolved in parallel with each polymerase. Results Extending previous in silico heuristics for the analysis of gene co-evolution, we analyzed the function of genes clustering with dnaE and polC . Clusters were highly informative. DnaE co-evolves with the ribosome, the transcription machinery, the core of intermediary metabolism enzymes. It is also connected to the energy-saving enzyme necessary for RNA degradation, polynucleotide phosphorylase. Most of the proteins of this co-evolving set belong to the persistent set in bacterial proteomes, that is fairly ubiquitously distributed. In contrast, PolC co-evolves with RNA degradation enzymes that are present only in the A+T-rich Firmicutes clade, suggesting at least two origins for the degradosome. Conclusion DNA replication involves two machineries, DnaE and PolC. DnaE co-evolves with the core functions of bacterial life. In contrast PolC co-evolves with a set of RNA degradation enzymes that does not derive from the degradosome identified in gamma-Proteobacteria. This suggests that at least two independent RNA degradation pathways existed in the progenote community at the end of the RNA genome world.
Fichier principal
Vignette du fichier
1471-2164-13-69.pdf (1.48 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04579011 , version 1 (17-05-2024)

Licence

Paternité

Identifiants

Citer

Stefan Engelen, David Vallenet, Claudine Médigue, Antoine Danchin. Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC. BMC Genomics, 2012, 13 (1), pp.69. ⟨10.1186/1471-2164-13-69⟩. ⟨hal-04579011⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More