Article Dans Une Revue International Journal of Systematic and Evolutionary Microbiology Année : 2014

Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon from soil and a member of the archaeal phylum Thaumarchaeota

Résumé

A mesophilic, neutrophilic and aerobic, ammonia-oxidizing archaeon, strain EN76 T , was isolated from garden soil in Vienna (Austria). Cells were irregular cocci with a diameter of 0.6–0.9 µm and possessed archaella and archaeal pili as cell appendages. Electron microscopy also indicated clearly discernible areas of high and low electron density, as well as tubule-like structures. Strain EN76 T had an S-layer with p3 symmetry, so far only reported for members of the Sulfolobales . Crenarchaeol was the major core lipid. The organism gained energy by oxidizing ammonia to nitrite aerobically, thereby fixing CO 2 , but growth depended on the addition of small amounts of organic acids. The optimal growth temperature was 42 °C and the optimal pH was 7.5, with ammonium and pyruvate concentrations of 2.6 and 1 mM, respectively. The genome of strain EN76 T had a DNA G+C content of 52.7 mol%. Phylogenetic analyses of 16S rRNA genes showed that strain EN76 T is affiliated with the recently proposed phylum Thaumarchaeota , sharing 85 % 16S rRNA gene sequence identity with the closest cultivated relative ‘ Candidatus Nitrosopumilus maritimus’ SCM1, a marine ammonia-oxidizing archaeon, and a maximum of 81 % 16S rRNA gene sequence identity with members of the phyla Crenarchaeota and Euryarchaeota and any of the other recently proposed phyla (e.g. ‘ Korarchaeota ’ and ‘ Aigarchaeota ’). We propose the name Nitrososphaera viennensis gen. nov., sp. nov. to accommodate strain EN76 T . The type strain of Nitrososphaera viennensis is strain EN76 T ( = DSM 26422 T = JMC 19564 T ) . Additionally, we propose the family Nitrososphaeraceae fam. nov., the order Nitrososphaerales ord. nov. and the class Nitrososphaeria classis nov.

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hal-04576562 , version 1 (15-05-2024)

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Michaela Stieglmeier, Andreas Klingl, Ricardo J E Alves, Simon K. -M. R Rittmann, Michael Melcher, et al.. Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon from soil and a member of the archaeal phylum Thaumarchaeota. International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (Pt_8), pp.2738 - 2752. ⟨10.1099/ijs.0.063172-0⟩. ⟨hal-04576562⟩
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