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Article Dans Une Revue European Journal of Organic Chemistry Année : 2020

Detergent‐Like Polymerizable Monomers: Synthesis, Physicochemical, and Biochemical Characterization

Résumé

Three monomers with a maltose polar head, an alkyl hydrogenated chain, and an acrylamide‐based polymerizable moiety were synthesized. The self‐assembly properties in aqueous solutions of these monomers were studied by means of isothermal titration calorimetry (ITC), surface tension (SFT) measurements, and dynamic light scattering (DLS), which indicated the formation of small micellar aggregates of about 6 nm diameter. The critical micellar concentration (CMC) was found to depend on the length of the alkyl chain and on the nature of the polymerizable moiety, ranging from 0.35 m m to ca. 10 m m . The monomers were found to solubilize phospholipid vesicles and to extract a broad range of proteins from Escherichia coli membranes. Finally, the extraction of two membrane proteins, namely, the full‐length, wild‐type human G‐protein‐coupled receptor (GPCR) adenosine A2A receptor (A 2A R) and the bacterial transporter AcrB was demonstrated.

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Chimie
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Dates et versions

hal-04567280 , version 1 (03-05-2024)

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Christophe Bonnet, Pierre Guillet, Florian Mahler, Sébastien Igonet, Sandro Keller, et al.. Detergent‐Like Polymerizable Monomers: Synthesis, Physicochemical, and Biochemical Characterization. European Journal of Organic Chemistry, 2020, 2020 (33), pp.5340-5349. ⟨10.1002/ejoc.202000540⟩. ⟨hal-04567280⟩
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