Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Antioxidants and Redox Signaling Année : 2016

Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome

Résumé

Abstract Production of reactive oxygen species (ROS) in the phagosome by the NADPH oxidase is critical for mammalian immune defense against microbial infections and phosphoinositides are important regulators in this process. Phosphoinositol 3-phosphate (PI(3)P) regulates ROS production at the phagosome via p40phox by an unknown mechanism. This study tested the hypothesis that PI(3)P controls ROS production by regulating the presence of p40phox and p67phox at the phagosomal membrane. Pharmacologic inhibition of PI(3)P synthesis at the phagosome decreased the ROS production both in differentiated PLB-985 cells and human neutrophils. It also releases p67phox, the key cytosolic subunit of the oxidase, and p40phox from the phagosome. The knockdown of the PI(3)P phosphatase MTM1 or Rubicon or both increases the level of PI(3)P at the phagosome. That increase enhances ROS production inside the phagosome and triggers an extended accumulation of p67phox at the phagosome. Furthermore, the overexpression of MTM1 at the phagosomal membrane induces the disappearance of PI(3)P from the phagosome and prevents sustained ROS production. In conclusion, PI(3)P, indeed, regulates ROS production by maintaining p40phox and p67phox at the phagosomal membrane.
Fichier non déposé

Dates et versions

hal-04561116 , version 1 (26-04-2024)

Identifiants

Citer

Laurent Nault, Leïla Bouchab, Sophie Dupré-Crochet, Oliver Nüsse, Marie Erard. Environmental Effects on Reactive Oxygen Species Detection—Learning from the Phagosome. Antioxidants and Redox Signaling, 2016, 25 (10), pp.564-576. ⟨10.1089/ars.2016.6747⟩. ⟨hal-04561116⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More