Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes
Ferran Nieto-Fabregat
(1)
,
Qian Zhu
(2)
,
Corinne Vivès
(3)
,
Yunqin Zhang
(4)
,
Angela Marseglia
(5)
,
Fabrizio Chiodo
(6)
,
Michel Thépaut
(3)
,
Diksha Rai
(7)
,
Suvarn Kulkarni
(7)
,
Flaviana Di Lorenzo
(8)
,
Antonio Molinaro
(9)
,
Roberta Marchetti
(9)
,
Franck Fieschi
(3)
,
Guozhi Xiao
(10)
,
Biao Yu
(4)
,
Alba Silipo
(9)
1
University of Naples Federico II = Università degli studi di Napoli Federico II
2 CAS - Chinese Academy of Sciences
3 IBS - UMR 5075 - Institut de biologie structurale
4 KIB - Kunming Institute of Botany [CAS]
5 Dipartimento di Scienze Chimiche
6 ICB - Istituto di Chimica Biomolecolare = Institute of Biomolecular Chemistry
7 IIT Bombay - Indian Institute of Technology Bombay
8 Department of Chemical Sciences, Complesso Universitario Monte Sant'Angelo
9 "Federico II" University of Naples Medical School
10 Department of Biomolecular Systems [Potsdam]
2 CAS - Chinese Academy of Sciences
3 IBS - UMR 5075 - Institut de biologie structurale
4 KIB - Kunming Institute of Botany [CAS]
5 Dipartimento di Scienze Chimiche
6 ICB - Istituto di Chimica Biomolecolare = Institute of Biomolecular Chemistry
7 IIT Bombay - Indian Institute of Technology Bombay
8 Department of Chemical Sciences, Complesso Universitario Monte Sant'Angelo
9 "Federico II" University of Naples Medical School
10 Department of Biomolecular Systems [Potsdam]
Ferran Nieto-Fabregat
- Fonction : Auteur
- PersonId : 1326609
- ORCID : 0000-0001-9847-3030
Angela Marseglia
- Fonction : Auteur
- PersonId : 1377740
- ORCID : 0000-0003-1831-6831
Suvarn Kulkarni
- Fonction : Auteur
- PersonId : 1377741
- ORCID : 0000-0003-2884-876X
Antonio Molinaro
- Fonction : Auteur
- PersonId : 1149740
- ORCID : 0000-0002-3456-7369
Roberta Marchetti
- Fonction : Auteur
- PersonId : 1291000
- ORCID : 0000-0002-7173-7099
Franck Fieschi
- Fonction : Auteur
- PersonId : 761070
- ORCID : 0000-0003-1194-8107
- IdRef : 087253240
Guozhi Xiao
- Fonction : Auteur
- PersonId : 1377742
- ORCID : 0000-0002-4724-4390
Biao Yu
- Fonction : Auteur
- PersonId : 1377743
- ORCID : 0000-0002-3607-578X
Alba Silipo
- Fonction : Auteur
- PersonId : 1326611
- ORCID : 0000-0002-5394-6532
Résumé
The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical dissection of BVMPK LPS components, attained by synthetic chemistry combined to spectroscopic, biophysical, and computational techniques, allowed to finely map the LPS epitopes recognized by DC-SIGN. Our findings reveal BVMPK's role in immune modulation via DC-SIGN, targeting both the LPS O-antigen and the core oligosaccharide. Furthermore, when framed within medical chemistry or drug design, our results could lead to the development of tailored molecules to benefit the hosts dealing with inflammatory diseases.