Obtaining of New Antioxidant and Antimicrobial Peptides Derived from Human Hemoglobin by Peptide Hydrolysis and Comparison with These Obtained by Bovine Hemoglobin - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2023

Obtaining of New Antioxidant and Antimicrobial Peptides Derived from Human Hemoglobin by Peptide Hydrolysis and Comparison with These Obtained by Bovine Hemoglobin

Résumé

This study focuses on the enzymatic hydrolysis of hemoglobin, the main component of cruor that gives blood its red color in mammals. The antibacterial and antioxidant potential of human hemoglobin hydrolysates were evaluated in comparison to bovine hemoglobin. The results showed strong antimicrobial activity of the peptide hydrolysates against six bacterial strains, independent of the initial substrate concentration level. The hydrolysates also showed strong antioxidant activity measured by four different tests. In addition, the antimicrobial and antioxidant activity of the human and bovine hemoglobin hydrolysates showed little or no significant difference, with only the concentration level being the determining factor in their activity. The results of the mass spectrometry study showed the presence of a number of bioactive peptides, the majority of which have characteristics similar to those mentioned in the literature. New bioactive peptides were also identified in human hemoglobin, such as antibacterial peptides PTTKTYFPHF (α37-46), FPTTKTYFPH (α36-45), TSKYR (α137-141) and STVLTSKYR (α133-141), as well as an antioxidant TSKYR (α137-141). According to these findings, human hemoglobin represents a promising source of bioactive peptides beneficial to the food or pharmaceutical industries.
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Dates et versions

hal-04551995 , version 1 (18-04-2024)

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Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Jihen Dhaouefi, et al.. Obtaining of New Antioxidant and Antimicrobial Peptides Derived from Human Hemoglobin by Peptide Hydrolysis and Comparison with These Obtained by Bovine Hemoglobin. International Journal of Molecular Sciences, 2023, ⟨10.20944/preprints202307.0286.v1⟩. ⟨hal-04551995⟩
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