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Article Dans Une Revue Nature Communications Année : 2024

Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer

Hugo Croizer
  • Fonction : Auteur
Rana Mhaidly
  • Fonction : Auteur
Yann Kieffer
Lounes Djerroudi
  • Fonction : Auteur
Renaud Leclere
Floriane Pelon
  • Fonction : Auteur
Catherine Robley
  • Fonction : Auteur
Mylene Bohec
Arnaud Meng
  • Fonction : Auteur
Didier Meseure
Emanuela Romano
Sylvain Baulande
Agathe Peltier
Anne Vincent-Salomon
Fatima Mechta-Grigoriou

Résumé

Abstract Although heterogeneity of FAP+ Cancer-Associated Fibroblasts (CAF) has been described in breast cancer, their plasticity and spatial distribution remain poorly understood. Here, we analyze trajectory inference, deconvolute spatial transcriptomics at single-cell level and perform functional assays to generate a high-resolution integrated map of breast cancer (BC), with a focus on inflammatory and myofibroblastic (iCAF/myCAF) FAP+ CAF clusters. We identify 10 spatially-organized FAP+ CAF-related cellular niches, called EcoCellTypes, which are differentially localized within tumors. Consistent with their spatial organization, cancer cells drive the transition of detoxification-associated iCAF (Detox-iCAF) towards immunosuppressive extracellular matrix (ECM)-producing myCAF (ECM-myCAF) via a DPP4- and YAP-dependent mechanism. In turn, ECM-myCAF polarize TREM2+ macrophages, regulatory NK and T cells to induce immunosuppressive EcoCellTypes, while Detox-iCAF are associated with FOLR2+ macrophages in an immuno-protective EcoCellType. FAP+ CAF subpopulations accumulate differently according to the invasive BC status and predict invasive recurrence of ductal carcinoma in situ (DCIS), which could help in identifying low-risk DCIS patients eligible for therapeutic de-escalation.

Dates et versions

hal-04546982 , version 1 (15-04-2024)

Identifiants

Citer

Hugo Croizer, Rana Mhaidly, Yann Kieffer, Geraldine Gentric, Lounes Djerroudi, et al.. Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer. Nature Communications, 2024, 15 (1), pp.2806. ⟨10.1038/s41467-024-47068-z⟩. ⟨hal-04546982⟩
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