An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Food Microbiology Année : 2024

An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue

Ingeborg L.A. Boxman
Ramia Molin
  • Fonction : Auteur
Sofia Persson
  • Fonction : Auteur
Anna Juréus
  • Fonction : Auteur
Claudia C.C. Jansen
  • Fonction : Auteur
Nils P. Sosef
  • Fonction : Auteur
Maija Summa
  • Fonction : Auteur
Maria Hautaniemi
  • Fonction : Auteur
Elisabetta Suffredini
  • Fonction : Auteur
Simona Di Pasquale
  • Fonction : Auteur
Mette Myrmel
  • Fonction : Auteur
Mamata Khatri
  • Fonction : Auteur
Urska Jamnikar-Ciglenecki
  • Fonction : Auteur
Darja Kusar
  • Fonction : Auteur
Dominik Moor
  • Fonction : Auteur
Lisa Butticaz
  • Fonction : Auteur
James A. Lowther
  • Fonction : Auteur
David I. Walker
  • Fonction : Auteur
Tina Stapleton
  • Fonction : Auteur
Magnus Simonsson
  • Fonction : Auteur
René A.M. Dirks
  • Fonction : Auteur

Résumé

An optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonucleotides, was used on a Bio-Rad QX200 platform by six laboratories. Adapted assays for Qiagen Qiacuity or ThermoFisher QuantStudio 3D were used by one laboratory each. The ILS comprised quantification of norovirus RNA in the absence of matrix and in oyster tissue samples. On average, results of the RT-dPCR assays were very similar to those obtained by RT-qPCR assays. The coefficient of var iation (CV%) of norovirus GI results was, however, much lower for RT-dPCR than for RT-qPCR in intra-laboratory replicates (eight runs) and between the eight laboratories. The CV% of norovirus GII results was in the same range for both detection formats. Had in-house prepared dsDNA standards been used, the CV% of norovirus GII could have been in favor of the RT-dPCR assay. The ratio between RT-dPCR and RT-qPCR results varied per laboratory, despite using the distributed RT-qPCR dsDNA standards. The study indicates that the RT-dPCR assay is likely to increase uniformity of quantitative results between laboratories.
Fichier non déposé

Dates et versions

hal-04540152 , version 1 (10-04-2024)

Identifiants

Citer

Ingeborg L.A. Boxman, Ramia Molin, Sofia Persson, Anna Juréus, Claudia C.C. Jansen, et al.. An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue. Food Microbiology, 2024, 120, 104478 (12p.). ⟨10.1016/j.fm.2024.104478⟩. ⟨hal-04540152⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More