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Article Dans Une Revue Veterinary Research Année : 2024

circRNA_8521 promotes Senecavirus A infection by sponging miRNA-324 to regulate LC3A

Xiwang Yang
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Rui Liu
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Yunsha Du
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Caiqiu Mei
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Guangneng Zhang
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Chen Wang
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Yijun Yang
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Zhiwen Xu
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Wenting Li
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Xiao Liu
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Résumé

AbstractSenecavirus A (SVA) causes outbreaks of vesicular disease in pigs, which imposes a considerable economic burden on the pork industry. As current SVA prevention measures are ineffective, new strategies for controlling SVA are urgently needed. Circular (circ)RNA is a newly characterized class of widely expressed, endogenous regulatory RNAs, which have been implicated in viral infection; however, whether circRNAs regulate SVA infection remains unknown. To investigate the influence of circRNAs on SVA infection in porcine kidney 15 (PK-15) cells, RNA sequencing technology was used to analyze the circRNA expression profiles of SVA-infected and uninfected PK-15 cells, the interactions between circRNAs, miRNAs, and mRNAs potentially implicated in SVA infection were predicted using bioinformatics tools. The prediction accuracy was verified using quantitative real-time (qRT)-PCR, Western blotting, as well as dual-luciferase reporter and RNA pull-down assays. The results showed that 67 circRNAs were differentially expressed as a result of SVA infection. We found that circ_8521 was significantly upregulated in SVA-infected PK-15 cells and promoted SVA infection. circ_8521 interacted with miR-324. miR-324 bound to LC3A mRNA which inhibited the expression of LC3A. Knockdown of LC3A inhibited SVA infection. However, circ_8521 promoted the expression of LC3A by binding to miR-324, thereby promoting SVA infection. We demonstrated that circ_8521 functioned as an endogenous miR-324 sponge to sequester miR-324, which promoted LC3A expression and ultimately SVA infection.
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Dates et versions

hal-04536146 , version 1 (08-04-2024)

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Xiwang Yang, Rui Liu, Yunsha Du, Caiqiu Mei, Guangneng Zhang, et al.. circRNA_8521 promotes Senecavirus A infection by sponging miRNA-324 to regulate LC3A. Veterinary Research, 2024, 55 (1), pp.43. ⟨10.1186/s13567-024-01291-0⟩. ⟨hal-04536146⟩

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