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Poster De Conférence Année : 2021

A novel network slow oscillation in the claustrum in vitro is driven by GABA-A rhythmic inhibition

Gilles R Ouanounou
Thierry Bal
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Résumé

The claustrum and the endopiriform nucleus form a complex with a sheet-like structure located in between the insular cortex and the striatum. It contains a variety of cell types, interneurons and far-projecting excitatory claustrocortical neurons, which makes it the most connected structure in the forebrain. Despite its early presence in amniotes including reptiles and mammals (Norimoto et al., Nature 2020), the functional role of this forebrain hub remains largely unknown. The claustrum has been hypothesized to mediate the unified sense of cognition (Crick and Koch, 2005), attention (Atlan et al., 2018), and may modulate the synchronization of neocortical slow-wave activity during sleep (Narikiyo et al., 2020). In a mouse brain slice that contains parts of the claustrum and the insular cortex, we found that claustral principal cells undergo a synchronized and regular 0,3-1 Hz network oscillation under application of the cholinergic agonist carbachol. Light-induced release of acetylcholine from channelrhodopsin-containing cholinergic afferents boosts the oscillation. In order to unravel the mechanisms of this oscillation we performed voltage-clamp protocols in neurons from wild-type and transgenic mice. In principal claustral neurons identified by their somatic shape and electrophysiological characteristics, we found a clear rhythmicity of inhibitions, while excitatory post-synaptic currents distribution remained mostly non-rhythmic. Rhythmic inhibitions were driven through chloride and not potassium conductances, suggesting the involvement of GABA-A receptors. In current-clamp, rhythmic burst firing of action potentials in principal cells was generated by excitatory inputs and terminated by inhibitions. These experiments suggest a pacemaker role for inhibitory neurons under the influence of cholinergic modulation of the network. The pacemaker interneurons within the claustrum remain to be identified, and possible synaptic loops between the claustrum and the adjacent insular cortex remains to be examined.
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Dates et versions

hal-04530856 , version 1 (03-04-2024)

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  • HAL Id : hal-04530856 , version 1

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Gilles R Ouanounou, Thierry Bal. A novel network slow oscillation in the claustrum in vitro is driven by GABA-A rhythmic inhibition. NeuroFrance 2021, Strasbourg International virtual meeting, May 2021, Strasbourg (100% virtuel), France. ⟨hal-04530856⟩
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