Enrichment and characterization of muscle extracellular vesicles (EVs) during Trichinella spiralis infection in mice - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2024

Enrichment and characterization of muscle extracellular vesicles (EVs) during Trichinella spiralis infection in mice

Résumé

During the muscle invasion by Trichinella spiralis newborn larvae (NBL), the transformation of muscle cells to nurse cells is still not understood, in particular the molecular interplays between host and parasite remains not identified. The emerging role of extracellular vesicles (EVs) in the latest decades opens a new field in the understanding of how the pathogen makes itself a niche indispensable for its survival. Produced by almost all cell types, it has been identified as a new way of cell-cell communication in mammals and a new way of host-pathogen interactions in helminths. Muscle cells themselves produce EVs during physiological mechanisms, like regeneration (to which infection with Trichinella has been compared), or pathological conditions, like denervation, inflammation or insulin resistance. In regeneration, muscle cells exchange EVs with satellite cells to induce their proliferation or with immune cells to induce an inflammatory response. Moreover, muscle EVs are enriched in vimentin, an intermediate filament protein. We have previously shown that a NBL stage specific protein (NBL-1) induce the expression of vimentin in muscle cells in vitro. We therefore investigated muscle EVs in OF1 mice during oral infection by Trichinella spiralis (ISS004). EVs were enriched by ultracentrifugation after muscle digestion and analysed for their size and number by tunable resistive pulse sensing (tRPS), purity through the absence of cell-associated proteins (immunodetection in Western Blot), EVs-associated markers (CD9, CD63, Tsg101) and the vimentin protein content. The presence of Trichinella antigens has also been tested. Muscle EVs properties vary during the infection cycle. Vimentin and CD9 protein content of EVs (normalised to EVs protein rate) varies. CD9 tetraspanin is also known as a regulator of cell fusion during muscle regeneration. These results will allow to initiate a more global analysis of local EVs in muscle by proteomic and transcriptomic to understand how the parasite and the cell populations interact through this way. New biomarker or vaccine target candidates may thus be identified.

Domaines

Parasitologie
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Dates et versions

hal-04530626 , version 1 (03-04-2024)

Identifiants

  • HAL Id : hal-04530626 , version 1

Citer

Marie Maurer, Elaa Chkhaydem, Amandine Blaizot, Laura-Lucia Estevez, Damien Destouches, et al.. Enrichment and characterization of muscle extracellular vesicles (EVs) during Trichinella spiralis infection in mice. 4ème journée Recherche de l'IUT, Mar 2024, Vitry Sur Seine, France. ⟨hal-04530626⟩
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