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Communication Dans Un Congrès Année : 2020

Synchrotron-based imaging reveals silver ions trafficking within hepatocytes exposed to silver nanoparticles

Résumé

The widespread use of silver nanoparticles (AgNP) in consumer goods raises concerns about their toxicity to humans and their impact on environment [1]. AgNP toxicity in cells and animals has been extensively studied and it has been shown that the toxicity depends upon the release of Ag(I) ions from the NP[2,3]. Besides, Ag accumulates in liver following AgNP exposure [4]. In this context, we studied AgNP internalization and fate into hepatocytes. We made use of a synchrotron nanoprobe to visualize the subcellular distribution of silver. The combined use of X-ray fluorescence (XRF) microscopy on whole cells and electron microscopy allowed the discrimination between the nanoparticle form located inside endosomes and lysosomes and the ionic species that distribute throughout the cell [5]. Besides, synchrotron X-ray absorption spectroscopy showed that Ag(I) recombines with sulphur in hepatocytes in the form of AgS2 and AgS3 complexes[5,6]. More recently, we developed a nano-XRF method performed on cell sections (Figure 1) that can be correlated with electron microscopy to reveal Ag(I) species distribution at the organelle level under long-term exposure to non-toxic concentration of AgNPs. We thus observed Ag(I) species in different organelles including in the nucleus [7]. This approach was also used on sections from 3D hepatic cell cultures that mimic liver architecture including bile canaliculi. XRF allowed to visualize Ag(I) excretion into these intercellular structures. To get more insights into the fate and effects of AgNPs, these data were completed with 3D electron microscopy, STEM-EDX and physiology assays. The later revealed, for the first time, that Ag(I) species translocating into the nucleus can trigger an endocrine disruptor-like effect. Overall, synchrotron-based imaging was central in our studies that aim at understanding the fate of nanomaterials in cells and organisms.
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hal-04522003 , version 1 (26-03-2024)

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  • HAL Id : hal-04522003 , version 1

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Aurélien Deniaud, Giulia Veronesi, Vanessa Tardillo Suàrez, Benoît Gallet, Mireille Chevallet, et al.. Synchrotron-based imaging reveals silver ions trafficking within hepatocytes exposed to silver nanoparticles. European Synchrotron Radiation Facility (ESRF) User Meeting 2020,Nanomaterials life cycle: from nanoengineering to public health; Session II - Nanomaterials and Human Health, Feb 2020, Grenoble, Institut de biologie structurale- (présentiel), France. ⟨hal-04522003⟩
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