Anti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International journal for parasitology. Drugs and drug resistance Année : 2022

Anti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi

Résumé

Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of Dicranum majus and Thuidium delicatulum inhibited NO production in a concentration-dependent manner with IC50 values of 1.04 and 1.54 µg/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.

Dates et versions

hal-04503144 , version 1 (13-03-2024)

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Miguel Peña-Espinoza, Yeambell Romero-Uzqueda, Angela Valente, Matthew de Roode, Henrik Toft Simonsen, et al.. Anti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi. International journal for parasitology. Drugs and drug resistance, 2022, 20 (6), pp.43-53. ⟨10.1016/j.ijpddr.2022.08.002⟩. ⟨hal-04503144⟩
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