Ambidexterity has become a major issue for public organizations as they manage increasingly strong contradictory pressures to optimize existing processes while innovating. Moreover, although public innovation laboratories are emerging, their influence on the development of ambidexterity remains largely unexplored. Our research aims to understand how innovation laboratories contribute to the formation of individual ambidexterity within the public sector. Drawing from three case studies, this research underscores the influence of these labs on public ambidexterity through the development of innovations by non-specialized actors and the deployment and reuse of innovative managerial practices and techniques outside the i-labs.