Rattus norvegicus Glutathione Transferase Omega 1 Localization in Oral Tissues and Interactions with Food Phytochemicals
Nicolas Poirier
(1)
,
Franck Ménétrier
(1)
,
Jade Moreno
(1)
,
Valentin Boichot
(1)
,
Jean-Marie Heydel
(1)
,
Claude Didierjean
(2)
,
Marie-Chantal Canivenc-Lavier
(1)
,
Francis Canon
(1)
,
F. Neiers
(1)
,
Mathieu Schwartz
(1)
Nicolas Poirier
- Fonction : Auteur
- PersonId : 1178258
- IdHAL : nipoirier
Valentin Boichot
- Fonction : Auteur
- PersonId : 1395155
- IdHAL : valentin-boichot
- ORCID : 0000-0002-7487-4590
Jean-Marie Heydel
- Fonction : Auteur
- PersonId : 1151034
- IdHAL : jean-marie-heydel
- ORCID : 0000-0002-6089-9922
- IdRef : 059796138
Claude Didierjean
- Fonction : Auteur
- PersonId : 14182
- IdHAL : claude-didierjean
- ORCID : 0000-0002-3176-8974
Marie-Chantal Canivenc-Lavier
- Fonction : Auteur
- PersonId : 1153797
- IdHAL : marie-chantal-canivenc-lavier
- ORCID : 0000-0001-8594-0576
Francis Canon
- Fonction : Auteur
- PersonId : 735990
- IdHAL : francis-canon
- ORCID : 0000-0002-6140-5007
- IdRef : 136738427
F. Neiers
- Fonction : Auteur
- PersonId : 740725
- IdHAL : fabrice-neiers
- ORCID : 0000-0002-8965-4929
- IdRef : 120126923
Mathieu Schwartz
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- Fonction : Auteur correspondant
- PersonId : 14190
- IdHAL : mathieu-schwartz
- ORCID : 0000-0002-2670-5274
- IdRef : 230806929
Connectez-vous pour contacter l'auteur
Résumé
Glutathione transferases are xenobiotic-metabolizing enzymes with both glutathione-conjugation and ligandin roles. GSTs are present in chemosensory tissues and fluids of the nasal/oral cavities where they protect tissues from exogenous compounds, including food molecules. In the present study, we explored the presence of the omega-class glutathione transferase (GSTO1) in the rat oral cavity. Using immunohistochemistry, GSTO1 expression was found in taste bud cells of the tongue epithelium and buccal cells of the oral epithelium. Buccal and lingual extracts exhibited thiol-transferase activity (4.9 ± 0.1 and 1.8 ± 0.1 μM/s/mg, respectively). A slight reduction from 4.9 ± 0.1 to 4.2 ± 0.1 μM/s/mg (p < 0.05; Student’s t test) was observed in the buccal extract with 100 μM GSTO1-IN-1, a specific inhibitor of GSTO1. RnGSTO1 exhibited the usual activities of omega GSTs, i.e., thiol-transferase (catalytic efficiency of 8.9 × 104 M–1·s–1), and phenacyl-glutathione reductase (catalytic efficiency of 8.9 × 105 M–1·s–1) activities, similar to human GSTO1. RnGSTO1 interacts with food phytochemicals, including bitter compounds such as luteolin (Ki = 3.3 ± 1.9 μM). Crystal structure analysis suggests that luteolin most probably binds to RnGSTO1 ligandin site. Our results suggest that GSTO1 could interact with food phytochemicals in the oral cavity.
Format du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Rattus norvegicus Glutathione Transferase Omega 1 Localization in Oral Tissues and Interactions with Food Phytochemicals
|
Résumé |
en
Glutathione transferases are xenobiotic-metabolizing enzymes with both glutathione-conjugation and ligandin roles. GSTs are present in chemosensory tissues and fluids of the nasal/oral cavities where they protect tissues from exogenous compounds, including food molecules. In the present study, we explored the presence of the omega-class glutathione transferase (GSTO1) in the rat oral cavity. Using immunohistochemistry, GSTO1 expression was found in taste bud cells of the tongue epithelium and buccal cells of the oral epithelium. Buccal and lingual extracts exhibited thiol-transferase activity (4.9 ± 0.1 and 1.8 ± 0.1 μM/s/mg, respectively). A slight reduction from 4.9 ± 0.1 to 4.2 ± 0.1 μM/s/mg (p < 0.05; Student’s t test) was observed in the buccal extract with 100 μM GSTO1-IN-1, a specific inhibitor of GSTO1. RnGSTO1 exhibited the usual activities of omega GSTs, i.e., thiol-transferase (catalytic efficiency of 8.9 × 104 M–1·s–1), and phenacyl-glutathione reductase (catalytic efficiency of 8.9 × 105 M–1·s–1) activities, similar to human GSTO1. RnGSTO1 interacts with food phytochemicals, including bitter compounds such as luteolin (Ki = 3.3 ± 1.9 μM). Crystal structure analysis suggests that luteolin most probably binds to RnGSTO1 ligandin site. Our results suggest that GSTO1 could interact with food phytochemicals in the oral cavity.
|
Auteur(s) |
Nicolas Poirier
1
, Franck Ménétrier
1
, Jade Moreno
1
, Valentin Boichot
1
, Jean-Marie Heydel
1
, Claude Didierjean
2
, Marie-Chantal Canivenc-Lavier
1
, Francis Canon
1
, F. Neiers
1
, Mathieu Schwartz
1
1
CSGA -
Centre des Sciences du Goût et de l'Alimentation [Dijon]
( 1096468 )
- Université de Bourgogne - Bât. CSG - 9E Bd Jeanne d'Arc - 21000 Dijon
- France
2
CRM2 -
Cristallographie, Résonance Magnétique et Modélisations
( 129683 )
- Université de Lorraine - Faculté des Sciences, boulevard des Aiguillettes, BP 70239, 54506 Vandoeuvre-les-Nancy Cedex
- France
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Langue du document |
Anglais
|
Nom de la revue |
|
Public visé |
Scientifique
|
Date de publication |
2024
|
Date de publication électronique |
2024-03-05
|
URL éditeur |
https://pubs.acs.org/doi/10.1021/acs.jafc.4c00483
|
Projet(s) ANR |
|
Domaine(s) |
|
Financement |
|
Voir aussi |
|
Mots-clés |
en
xenobiotic-metabolizing enzymes, taste, glutathione transferase, GST omega, oral cavity, flavonoids
|
DOI | 10.1021/acs.jafc.4c00483 |
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