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              <p>In the interest of constantly improving their diagnoses, the experts in forensic anthropology ofthe french Gendarmerie Nationale are interested in new evaluation methods to estimate post-mortem interval (PMI) of human bone. Indeed, currently used physico-chemical methodsbased on Nile Blue colorimetry allow to estimate PMI up to 30 years. However, the prescriptionperiod is 20 years and the desired estimation must be less than this delay.The development of non-destructive methods would allow coupling PMI estimations ofdifferent methods without alteration of the sample and thus improving accuracy. There areseveral studies validating the use of ultrasonic waves for the structural characterization ofbones in medical applications. The objective of this work is therefore the implementation ofnon-destructive ultrasonic evaluation techniques to determine whether wave parametersallowing the estimation of PMI emerge from structural variations between individuals.Firstly, this paper studies the wave propagation along the main axis of the bone. Incollaboration with the Institut de Recherche Criminelle de la Gendarmerie Nationale (IRCGN),the experiments were performed on bone samples with different PMI using (1) contacttransducers (1 MHz center frequency) for the measurement of acoustic signature intransmission mode and (2) a laser vibrometer for monitoring the propagation of the generatedwaves. Preliminary results show that the acoustic signatures make possible to monitor thestructural changes of bone.Secondly, the problem of extracting relevant characteristic parameters of bones arises whentaking into account the composition and the internal architecture that change from oneindividual to another and for which storage conditions and ageing are variable. Statistical studyon a large number of individuals should make it possible to define the robustness of theparameters selected for the estimation of the PMI.</p>
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