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Article Dans Une Revue Journal of Membrane Biology Année : 1994

Characterization of an angiotensin-II-activated chloride current in rabbit sino-atrial cells

Résumé

Single sino-atrial cells from rabbit heart were voltage-clamped using the whole-cell configuration of the patch clamp technique under conditions in which most of the ionic and exchange currents known in pacemaker cardiac cells were minimized. Extracellular angiotensin II (AII) activated a time-independent background current. The current-voltage relation of this current showed an outward rectification. The reversal potential was -20 mV with 156 mM Cl- external solution and 54 mM Cl- internal solution. This reversal potential shifted with changes in the transmembrane Cl- gradient in the fashion expected for a chloride current. Anthracene-9-carboxylic acid and diphenylamine-2-carboxylic acid (chloride channels blockers) were found to be effective in blocking the AII-sensitive current. The linear segment of the current-voltage relation can be totally inhibited by the competitive AII-receptor (AT1) antagonist losartan and by the presence of intracellular protein kinase C inhibitor, whereas the outward rectification is only slightly changed. It is concluded that sino-atrial cells should contain protein-kinase-C-sensitive chloride channels which may be activated by angiotensin II via the stimulation of the AT1 receptors.

Dates et versions

hal-04483660 , version 1 (29-02-2024)

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Citer

J. Bescond, P. Bois, J. Petit-Jacques, J. Lenfant. Characterization of an angiotensin-II-activated chloride current in rabbit sino-atrial cells. Journal of Membrane Biology, 1994, 140 (2), ⟨10.1007/BF00232903⟩. ⟨hal-04483660⟩

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