Digestive disorders occupy the second place of the major health problems in Burkina Faso. The fruits of Diospyros mespiliformis are used in recipes to treat gastroenteritis and gastroduodenal ulcer. Phytonutrients content was evaluated by specific reagents and biological activities by antiradical and antibacterial test. The fruit seed of Diospyros mespiliformis had the best antioxidant activity (IC 50 = 0.04 mg/mL) due to its content of total phenolic compounds (5.75 ± 0.6 mg GAE/g), flavonoids (0.78 ± 0.27 mg RE/g), hydrolyzable and condensed tannins (3.56 ± 0.03 mg TAE/g; 1.28 ± 0.42 mg CE/g) compared to the pulp whose content were 3.36 ± 0.12 mg GAE/g ; 0.46 ± 0.26 mg RE/g; 1.78 ± 0.35 mg TAE/g and 0.56 ± 0.11 mg CE/g respectively for total phenolic, flavonoids, hydrolysable and condensed tannins. The fruit seed had the best content of macronutrients (Na, K, Mg) and showed important antibacterial activity against E. coli. The fruit pulp had the best content of vitamin C, -carotene and vitamin E, being 22.0 ± 0.01mg/100g; 0.007 ± 0.03 mg/100g and 0.119 ± 0.02 mg/100g respectively. The results constitute scientific prerequisites for the development of new functional foods and phytomedicines in the management of digestive disorders.