Course Objectives This course aims to provide participants with the tools to do their best with their MALDI instruments: methodology for new MALDI applications and analytical tools for spectra analysis. The attendees will have the opportunity to interact with leading researchers in the field of MALDI-TOF massspectrometry applications in clinical microbiology and discuss recent breakthroughs and new applications. The workshop will include theoretical presentations and practical training, and will represent an excellent chance for networking between clinical microbiologists, basic researchers, MALDI experts, bioinformaticians and others.