Unsuccessful reproduction of Experimental Autoimmune Neuritis (EAN) in C57BL/6J mice using published protocol and variations
Résumé
This study aimed to establish an experimental model of Guillain-Barré Syndrome (GBS), a peripheral inflammatory neuropathy in humans, using the Experimental Autoimmune Neuritis (EAN) model in C57BL/6 mice. This approach was informed by a comprehensive bibliographic analysis. While this experimental model has been developed successfully in rabbits, rats, and the SJL mouse strain, the literature on its application in C57BL/6 mice is limited. Our protocol analysis encompassed twenty-one articles utilizing the P0180-199 neurogenic peptide to induce EAN in this genetic background. Based on this literature, we selected one protocol for replication and made three adaptations. Despite using high-quality P0180-199 peptide, we were unable to reproduce the selected EAN protocol or induce any pathological signs, even under optimized conditions. As a control to validate our reagents and methodology, we replicated a different model of inflammatory neuropathy targeting the central nervous system: the well-established Experimental Autoimmune Encephalitis (EAE) protocol. This replication was successful using the same C57BL/6 mouse genetic background and reagents, with the sole exception of the neurogenic peptide employed (MOG35-55).
Mots clés
Categories: Nature: negative result repetition/replication Model: in vivo Specie: mouse Data type: methods neuroinflammation autoimmunity demyelination
Categories: Nature: negative result
repetition/replication Model: in vivo Specie: mouse Data type: methods neuroinflammation
autoimmunity
demyelination
Domaines
Sciences du Vivant [q-bio]
Fichier principal
SN1-Unsuccessful reproduction of Experimental Autoimmune Neuritis-SN1_short-note.pdf (365.53 Ko)
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SN1-S1 supplemental Table-Bibliographic analysis review table of EAN protocols articles 4-24 (2).xlsx (2.93 Mo)
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SN1-S2 Supdata-ProteoGenix QC analysis P0 180-199 EAN.pdf (796.5 Ko)
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SN1-S3a Supdata-CBM QC analysis P0 ProteoGenix MS report fr.pdf (584.99 Ko)
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SN1-S3b Supdata-CBM QC analysis P0 ProteoGenix LC-UV-MS report fr.pdf (409.69 Ko)
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SN1-S4 Supdata-CBM QC analysis P0 MCE MS LC-UV-MS report fr.pdf (543.76 Ko)
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SN1-S5 Supdata-S3a-3b-4 CBM QC reports translated.pdf (1.5 Mo)
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SN1-S6 Supdata-Raw data for weights and clinical scores.xlsx (24.41 Ko)
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