L3MBTL2 protein acts in concert with PcG protein-mediated monoubiquitination of H2A to establish a repressive chromatin structure - Archive ouverte HAL
Article Dans Une Revue Molecular Cell Année : 2011

L3MBTL2 protein acts in concert with PcG protein-mediated monoubiquitination of H2A to establish a repressive chromatin structure

Patrick Trojer
  • Fonction : Auteur
Alina R. Cao
  • Fonction : Auteur
Zhonghua Gao
  • Fonction : Auteur
Yan Li
  • Fonction : Auteur
Jin Zhang
  • Fonction : Auteur
Xiaoqin Xu
  • Fonction : Auteur
Guohong Li
  • Fonction : Auteur
Hediye Erdjument-Bromage
  • Fonction : Auteur
Paul Tempst
  • Fonction : Auteur
Peggy J. Farnham
  • Fonction : Auteur
Danny Reinberg
  • Fonction : Auteur

Résumé

We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1gamma, known to function in transcriptional repression. PRC1L4-mediated repression necessitated L3MBTL2 that compacted chromatin in a histone modification-independent manner. Genome-wide location analyses identified several hundred genes simultaneously bound by L3MBTL2 and E2F6, preferentially around transcriptional start sites that exhibited little overlap with those targeted by other E2Fs or by L3MBTL1, another MBT domain-containing protein that interacts with RB1. L3MBTL2-specific RNAi resulted in increased expression of target genes that exhibited a significant reduction in H2A lysine 119 monoubiquitination. Our findings highlight a PcG/MBT collaboration that attains repressive chromatin without entailing histone lysine methylation marks.

Dates et versions

hal-04471991 , version 1 (21-02-2024)

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Citer

Patrick Trojer, Alina R. Cao, Zhonghua Gao, Yan Li, Jin Zhang, et al.. L3MBTL2 protein acts in concert with PcG protein-mediated monoubiquitination of H2A to establish a repressive chromatin structure. Molecular Cell, 2011, 42 (4), pp.438-450. ⟨10.1016/j.molcel.2011.04.004⟩. ⟨hal-04471991⟩
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