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Article Dans Une Revue Nature Communications Année : 2024

A microfluidic platform integrating functional vascularized organoids-on-chip

Clément Quintard
  • Fonction : Auteur
Gustav Jonsson
Jie Jiao
  • Fonction : Auteur
Jun Wang
Nicolas Werschler
Camille Laporte
Thierno-Sidy Bah
  • Fonction : Auteur
Gideon Pomeranz
  • Fonction : Auteur
Caroline Bissardon
  • Fonction : Auteur
Joris Kaal
  • Fonction : Auteur
Alexandra Leopoldi
  • Fonction : Auteur
David Long
Pierre Blandin
  • Fonction : Auteur
Jean-Luc Achard
  • Fonction : Auteur
Christophe Battail
Astrid Hagelkruys
Fabrice Navarro
  • Fonction : Auteur
Yves Fouillet
  • Fonction : Auteur
Josef Penninger

Résumé

Abstract The development of vascular networks in microfluidic chips is crucial for the long-term culture of three-dimensional cell aggregates such as spheroids, organoids, tumoroids, or tissue explants. Despite rapid advancement in microvascular network systems and organoid technologies, vascularizing organoids-on-chips remains a challenge in tissue engineering. Most existing microfluidic devices poorly reflect the complexity of in vivo flows and require complex technical set-ups. Considering these constraints, we develop a platform to establish and monitor the formation of endothelial networks around mesenchymal and pancreatic islet spheroids, as well as blood vessel organoids generated from pluripotent stem cells, cultured for up to 30 days on-chip. We show that these networks establish functional connections with the endothelium-rich spheroids and vascular organoids, as they successfully provide intravascular perfusion to these structures. We find that organoid growth, maturation, and function are enhanced when cultured on-chip using our vascularization method. This microphysiological system represents a viable organ-on-chip model to vascularize diverse biological 3D tissues and sets the stage to establish organoid perfusions using advanced microfluidics.

Dates et versions

hal-04465079 , version 1 (19-02-2024)

Identifiants

Citer

Clément Quintard, Emily Tubbs, Gustav Jonsson, Jie Jiao, Jun Wang, et al.. A microfluidic platform integrating functional vascularized organoids-on-chip. Nature Communications, 2024, 15 (1), pp.1452. ⟨10.1038/s41467-024-45710-4⟩. ⟨hal-04465079⟩
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