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Poster De Conférence Année : 2019

Investigating the involvement of the chromatin state in the control of gene expression in Leptospharia maculans

Résumé

Leptosphaeria maculans, a hemibiotrophic fungus responsible for stem canker, colonises oilseed rape in two stages: an early stage of cotyledon or leaf colonisation, and a late colonisation stage during which the fungus colonises systemically without visible symptom the plant before stem canker appears. L. maculans presents a bipartite genome structure alternating gene-rich and transposable element (TE)-rich regions. While TE-rich regions are enriched in putative effectorencoding genes strongly over-expressed during early infection ('early' effectors), gene-rich regions contain putative effector-encoding genes specifically expressed during late infection ('late' effectors). Here, we analyzed nucleosome positioning, chromatin structure and gene expression at the genome scale combining MAINE-seq, ChIP-seq and RNA-seq data during axenic growth and performed functional analysis of two players of heterochromatin assembly (KMT1 and KMT6). We analyzed in vitro ChIP-seq data targeting two heterochromatin modifications, H3K9me3 and H3K27me3, and a euchromatin modification, H3K4me2, and found that gene-rich regions are associated with H3K4me2 and H3K27me3 while TE-rich regions are associated with H3K9me3. Analysis of in vitro MAINE-seq data showed distinct nucleosome organization for genes located in TE-rich or gene-rich regions, and according to gene expression level. CRISPR-Cas9 mutants of KMT1 and KMT6 have been generated and are currently characterized for their ability to infect leaves and stems of oilseed rape, and to express effector genes.
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Dates et versions

hal-04456403 , version 1 (27-03-2024)

Identifiants

  • HAL Id : hal-04456403 , version 1

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Colin Clairet, Jessica L. Soyer, Elise Gay, Nicolas Lapalu, Adeline Simon, et al.. Investigating the involvement of the chromatin state in the control of gene expression in Leptospharia maculans. Fungal Genetics Conference, Mar 2019, Asiomar, United States. ⟨hal-04456403⟩
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