Overview of results
31 candidates found.
Get results as
chr |
position |
strand |
miRNA |
length |
weight |
reads |
reads distribution |
mfei |
alignment |
1 |
6,454,664-6,454,931 |
- |
UUAGAGAUUUUAGUUGGUUUAAUU |
24 |
44 |
204 |
★ |
-0.82 |
✓✓ |
1 |
6,514,677-6,514,930 |
+ |
CUUAUUUACAAUGUCAUGCUACU |
23 |
27 |
35 |
★ |
-0.92 |
✓ |
1 |
6,538,400-6,538,487 |
- |
AGAUGAUGGGCUUAGAUGAUGGGC |
24 |
380 |
1210 |
★★★ |
-0.76 |
|
1 |
6,538,703-6,538,797 |
+ |
AUAGAUCGGUAAAAGCAUGUUUUC |
24 |
4 |
11 |
★ |
-0.85 |
|
1 |
6,543,242-6,543,337 |
+ |
|
|
|
24 |
|
-0.86 |
|
1 |
6,543,291-6,543,423 |
+ |
|
|
|
261 |
|
-0.7 |
|
1 |
6,543,374-6,543,768 |
- |
AGAUGAUGGGCUUAGAUGAUGGGC |
24 |
380 |
1376 |
|
-0.72 |
|
1 |
6,592,984-6,593,131 |
+ |
AAAGAUAAAGCGGAUUAAACUGUU |
24 |
12 |
22 |
★★ |
-0.63 |
|
4 |
50,512-50,681 |
+ |
|
|
|
21 |
|
-0.89 |
|
4 |
77,043-77,331 |
- |
|
|
|
213 |
|
-0.77 |
|
4 |
77,276-77,585 |
+ |
|
|
|
103 |
|
-0.82 |
✓ |
4 |
297,901-298,116 |
- |
|
|
|
301 |
|
-0.79 |
|
4 |
298,193-298,561 |
- |
AAGCAGAACCCAAGAACAUAGUUU |
24 |
176 |
300 |
|
-0.77 |
|
4 |
313,266-313,379 |
- |
CUUGGUAUAUUCAAAUUAGGAUU |
23 |
11 |
36 |
|
-0.7 |
|
4 |
434,304-434,483 |
+ |
AGUGACCAUUGGAGCAUCGUGCCU |
24 |
72 |
152 |
★★★ |
-1.32 |
✓ |
4 |
618,259-618,526 |
- |
|
|
|
119 |
|
-1.26 |
|
4 |
743,281-743,392 |
- |
AUUUAGGGAAUCUAGUUUUUACUC |
24 |
19 |
25 |
★ |
-0.78 |
|
4 |
743,386-743,470 |
- |
AAGGUCUUGAAUGUUUUGUAGAUU |
24 |
31 |
44 |
★★ |
-1.26 |
|
4 |
983,183-983,345 |
- |
|
|
|
247 |
|
-0.81 |
✓ |
4 |
983,292-983,369 |
+ |
AUGUAAUGAUGGCUUGUAGAGAGU |
24 |
279 |
567 |
★ |
-0.92 |
|
4 |
985,565-985,737 |
+ |
GAGAGAGAGAGAGAGAGAGAGAG |
23 |
0.12 |
10 |
★★ |
-0.76 |
|
4 |
1,001,115-1,001,209 |
+ |
|
|
|
164 |
|
-0.83 |
|
4 |
1,023,847-1,024,244 |
- |
|
|
|
364 |
|
-1.59 |
✓ |
4 |
1,116,570-1,116,885 |
- |
ACGAAUCAAACAGUGAAAAGUGAC |
24 |
302 |
1438 |
★★ |
-1.69 |
✓✓ |
4 |
1,190,061-1,190,301 |
- |
|
|
|
101 |
|
-0.98 |
✓ |
4 |
1,212,018-1,212,144 |
+ |
|
|
|
94 |
|
-1.57 |
|
4 |
1,230,915-1,231,313 |
- |
AUCACAAUCGCGGUACAGUAAACG |
24 |
7 |
36 |
★★ |
-0.99 |
|
4 |
1,231,264-1,231,345 |
+ |
AAACGCGGAUUUGUGGUUAAUCUC |
24 |
24 |
69 |
★ |
-0.67 |
|
4 |
1,270,783-1,270,953 |
- |
CGGAGAAGCUGUAGGCGCUUUAUC |
24 |
13 |
66 |
★★ |
-0.62 |
|
4 |
1,438,780-1,438,882 |
+ |
UGUCCGUUGGAGAUUCUAUUGCCU |
24 |
7 |
17 |
★ |
-0.89 |
|
4 |
1,439,203-1,439,307 |
- |
|
|
|
98 |
|
-0.81 |
|
-
Chromosome: 1
-
Position: 6,454,664-6,454,931 (268 nt)
-
Strand: -
-
G+C content: 30.60 %
- miRNA sequence: UUAGAGAUUUUAGUUGGUUUAAUU (24 nt)
- miRNA depth: 44 (weight: 44)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -23.95 kcal/mol |
AMFE -25.11 |
MFEI -0.82
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 204 [download]
- Distribution of reads: one island ★
((((.(((..((((((((.(((....(((((((..(((((((.((((((((((.((.....((((((((((((((((.........(((((.........................))))).........))))))))))))))))....(((((((((..........)))))))))....(((((((((........))))))))))).))))))))))))))))))))))))...)))...))......))))))..))).))))
GUUAAACUUAAAUUGGCAUUGGUUUAGAGAUUUUAGUUGGUUUAAUUCAAUUUUACAAAACUUGAUGGGUUAAUUGGGUAAACCAUUGAAACCAUUAACCAUUACAACCUAACUUAUUUUAUUCAUCAAACCAAUUGACUCAUCAAAUCAUUUGACUCAUCAACUCAUUUGAGUCAAAAUUUUCAACUCAUUACGGUUUAUGGGUUGAGUUGAAUUGAGUUGACCAAUGAAUUUUGACCCAUUUUGAUACCCCUAAUUGUAGUAUAGC
((((.(((..((((((((.(((....(((((((..(((((((.((((((((((.((........................................................................................................................................................)).))))))))))))))))))))))))...)))...))......))))))..))).))))
...[[[[[[[..[[[[[[[.[[[[ ]]]]]]]]]]]]]]]]]]..
<-------miRBase-------->
........************************............................................................................................................................................................................................................................................ length=24 depth=2
.................************************................................................................................................................................................................................................................................... length=24 depth=7
.....................***********************................................................................................................................................................................................................................................ length=23 depth=2
......................************************.............................................................................................................................................................................................................................. length=24 depth=2
.......................***********************.............................................................................................................................................................................................................................. length=23 depth=2
.......................UUAGAGAUUUUAGUUGGUUUAAUU............................................................................................................................................................................................................................. length=24 depth=44
........................***********************............................................................................................................................................................................................................................. length=23 depth=22
........................************************............................................................................................................................................................................................................................ length=24 depth=7
.........................**********************............................................................................................................................................................................................................................. length=22 depth=5
.........................***********************............................................................................................................................................................................................................................ length=23 depth=4
.........................************************........................................................................................................................................................................................................................... length=24 depth=2
..........................***********************........................................................................................................................................................................................................................... length=23 depth=2
...............................................................................************************..................................................................................................................................................................... length=24 depth=4
................................................................................***********************..................................................................................................................................................................... length=23 depth=3
....................................................................................************************................................................................................................................................................................ length=24 depth=6
....................................................................................*************************............................................................................................................................................................... length=25 depth=2
.................................................................................................................................................************************................................................................................................... length=24 depth=3
......................................................................................................................................................************************.............................................................................................. length=24 depth=4
.......................................................................................................................................................*********************................................................................................................ length=21 depth=2
.......................................................................................................................................................................................................**********************............................................... length=22 depth=2
.......................................................................................................................................................................................................************************............................................. length=24 depth=2
.......................................................................................................................................................................................................................************************............................. length=24 depth=7
..........................................................................................................................................................................................................................***********************........................... length=23 depth=2
..........................................................................................................................................................................................................................************************.......................... length=24 depth=14
...........................................................................................................................................................................................................................***********************.......................... length=23 depth=5
...........................................................................................................................................................................................................................************************......................... length=24 depth=2
............................................................................................................................................................................................................................**********************.......................... length=22 depth=5
............................................................................................................................................................................................................................***********************......................... length=23 depth=3
miRBase alignment: ✓✓ presence of alignments that cover the miRNA locus (see reads cloud above)
Prediction : 201-224
A UA ------ U-- UUUA UA U U AAAACUUGAUGGGUUAAUUGGGUAAACCAUUGAAACCAUUAACCAUUACAACCUAACUUAUUUUAUUCAUCAAAC
GUUA ACU AAUUGG CA UGG GAGAUUU GUUGGUU AAUUCAAUUU AC C
|||| ||| |||||| || ||| ||||||| ||||||| |||||||||| ||
CGAU UGA UUAAUC GU ACC UUUUAAG UAACCAG UUGAGUUAAG UG A
A UG CCCAUA UUU CAG- -- - U AGUUGGGUAUUUGGCAUUACUCAACUUUUAAAACUGAGUUUACUCAACUACUCAGUUUACUAAACUACUCAGUUA
Alignments
query 201 UGGGUUGAGUUGAAUUGAGUUGAC 224
miRBase 1 UGGGUUGAGUUGAGUUGAGUUGGC
************* ******** *
miRBase 1 : MIMAT0022425 |
-
Chromosome: 1
-
Position: 6,514,677-6,514,930 (254 nt)
-
Strand: +
-
G+C content: 16.14 %
- miRNA sequence: CUUAUUUACAAUGUCAUGCUACU (23 nt)
- miRNA depth: 27 (weight: 27)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -7.12 kcal/mol |
AMFE -14.80 |
MFEI -0.92
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 35 [download]
- Distribution of reads: one island ★
((((.....(((.(((((((.(((((((.(((((((((((...((((((..((((.......((((..(((((((....)))))))..))))....)))).)))))).....(((((((..................))))))).....(((((.(((.(((.(((...(((((((........)))))))...))).))).))).))))).))))))))))).)))..)))).))))))).))).....))))
GUAUUUUGUUAACAAUAUUUUAAAAUAAAGUUUUUUGUUUAAAUAAAUAUUGUUCUUACUAAACGUUUUUUGUUUAAAUAAACAAAUCAUGUAUUUGAACUUAUUUACAAUGUCAUGCUACUGACAUUAAUUAUUUAGGCAUGAAAAUAUUUAAUUAAAGAUUUAAAUCUUCUAAAUUCUUGCUUUUAGAGUUAUUAUAUCAUUAUUUAAAGAGGCAAAAAAUAUUAAUUUUUUAAAUAUUAUUAUAUAUAUAU
((((.....(((.(((((((.(((((((.(((((((((((............................................................................................................................................................................))))))))))).)))..)))).))))))).))).....))))
<------miRBase------>
...............................................................................................************************....................................................................................................................................... length=24 depth=2
................................................................................................***********************....................................................................................................................................... length=23 depth=2
...................................................................................................CUUAUUUACAAUGUCAUGCUACU.................................................................................................................................... length=23 depth=27
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 153-173
UUUGU C U -- A AAAUAAAUAUUGUUCUUACUAAACGUUUUUUGUUUAAAUAAACAAAUCAUGUAUUUGAACUUAUUUACAAUGUCAUGCUACUGAC
GUAU UAA AAUAUUU AAAA UAA GUUUUUUGUUU A
|||| ||| ||||||| |||| ||| |||||||||||
UAUA AUU UUAUAAA UUUU AUU UAAAAAACGGA U
UAUAU A U UA A GAAAUUUAUUACUAUAUUAUUGAGAUUUUCGUUCUUAAAUCUUCUAAAUUUAGAAAUUAAUUUAUAAAAGUACGGAUUUAUUAAU
Alignments
query 153 AAUUAAAGAUUUAAAUCUU-CU 173
miRBase 1 AAUUAAAGAUUU-CAUCUUACU
************ ***** **
miRBase 1 : MIMAT0013773 |
-
Chromosome: 1
-
Position: 6,538,400-6,538,487 (88 nt)
-
Strand: -
-
G+C content: 38.64 %
- miRNA sequence: AGAUGAUGGGCUUAGAUGAUGGGC (24 nt)
- miRNA depth: 760 (weight: 380)
- Other precursors with the same miRNA: 1__6543374-6543768
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -25.80 kcal/mol |
AMFE -29.32 |
MFEI -0.76
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 1210 [download]
- Distribution of reads: two islands ★★
CCCUAGAAACAUCUAGAUGAUGGGCUUAGAUGAUGGGCCUUAUAAUGGGCAUCCAUCUUUAUUCAUAACAUUUUUUUUGUUGUUGGGG
((((((.((((...((((((((((..((((.(((((((((......))))..))))))))))))))..)))))....)))).))))))
[[[[[[[[[[..[[[[.[[[[[[[ ]]..]]]]]]]]]]]]]]..]]]]]
.*************************.............................................................. length=25 depth=15
..************************.............................................................. length=24 depth=66
...************************............................................................. length=24 depth=28
....***********************............................................................. length=23 depth=16
......************************.......................................................... length=24 depth=15
..........************************...................................................... length=24 depth=32
............************************.................................................... length=24 depth=16
..............AGAUGAUGGGCUUAGAUGAUGGGC.................................................. length=24 depth=760
...............***********************.................................................. length=23 depth=27
...............************************................................................. length=24 depth=17
..................*********************................................................. length=21 depth=32
miRBase alignment: none
-
Chromosome: 1
-
Position: 6,538,703-6,538,797 (95 nt)
-
Strand: +
-
G+C content: 27.37 %
- miRNA sequence: AUAGAUCGGUAAAAGCAUGUUUUC (24 nt)
- miRNA depth: 4 (weight: 4)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -22.00 kcal/mol |
AMFE -23.16 |
MFEI -0.85
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 11 [download]
- Distribution of reads: random
GAUCGAUGAUUAUAGUAUAGAUCGGUAAAAGCAUGUUUUCAAUGAUUCAAUAAAGAAGAGAAUCAUGCUUAUCAUUUGUUUCUAUAAUUACUAUC
(((.(.(((((((((.((((((.((((..(((((((((((.....(((......)))))))).))))))))))))))))..)))))))))).)))
[[[[[[.[[[[..[[[[[[[[[[[ ]]]]].]]]]]]]]]]]]]]]]
..............******************............................................................... length=18 depth=2
................AUAGAUCGGUAAAAGCAUGUUUUC....................................................... length=24 depth=4
miRBase alignment: none
-
Chromosome: 1
-
Position: 6,543,242-6,543,337 (96 nt)
-
Strand: +
-
G+C content: 36.46 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -30.00 kcal/mol |
AMFE -31.25 |
MFEI -0.86
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 24 [download]
- Distribution of reads: random
GAGUAAUUGUAUGUUGCACUCUUUUUCCCUUAACCAUGGUUUUUCCCAUUGGGUUUUCCUGGUAAGGUUUUUAAUGAGGCAGCAUUUCAAAUACUU
(((((.(((.(((((((.(((.((...(((((.(((.((.....(((...)))....)))))))))).....)).))))))))))..))).)))))
..........************************.............................................................. length=24 depth=7
...........................................************************............................. length=24 depth=3
..............................................................*******************............... length=19 depth=2
miRBase alignment: none
-
Chromosome: 1
-
Position: 6,543,291-6,543,423 (133 nt)
-
Strand: +
-
G+C content: 39.10 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -36.40 kcal/mol |
AMFE -27.37 |
MFEI -0.7
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 261 [download]
- Distribution of reads: random
UGGGUUUUCCUGGUAAGGUUUUUAAUGAGGCAGCAUUUCAAAUACUUUACGAGUUAUGAUACUAUAAUGGUCAUCAGGGGGAGUGUUAUGAAUAAAGAUGGAUGCCCAUUAUAAGGCCCAUCAUCUAAGCCCA
(((((((...((((..(((((((((((.((((.((((((((((((((..(..(..(((((.(......)))))))..)..))))))).))).....))))..))))))))).)))))).))))...)))))))
................................................************************............................................................. length=24 depth=5
............................................................************************................................................. length=24 depth=7
.......................................................................***********************....................................... length=23 depth=4
...........................................................................************************.................................. length=24 depth=8
.............................................................................************************................................ length=24 depth=7
.................................................................................***********************............................. length=23 depth=3
.................................................................................************************............................ length=24 depth=15
..................................................................................************************........................... length=24 depth=39
...................................................................................***********************........................... length=23 depth=4
....................................................................................**********************........................... length=22 depth=14
....................................................................................***********************.......................... length=23 depth=4
....................................................................................************************......................... length=24 depth=4
.....................................................................................*********************........................... length=21 depth=18
.....................................................................................************************........................ length=24 depth=3
......................................................................................************************....................... length=24 depth=3
.......................................................................................*******************........................... length=19 depth=4
.......................................................................................*********************......................... length=21 depth=3
.......................................................................................***********************....................... length=23 depth=4
.............................................................................................************************................ length=24 depth=18
..............................................................................................***********************................ length=23 depth=5
..............................................................................................************************............... length=24 depth=4
...............................................................................................************************.............. length=24 depth=3
.....................................................................................................************************........ length=24 depth=8
..........................................................................................................************************... length=24 depth=5
...........................................................................................................***********************... length=23 depth=5
miRBase alignment: none
-
Chromosome: 1
-
Position: 6,543,374-6,543,768 (395 nt)
-
Strand: -
-
G+C content: 37.97 %
- miRNA sequence: AGAUGAUGGGCUUAGAUGAUGGGC (24 nt)
- miRNA depth: 760 (weight: 380)
- Other precursors with the same miRNA: 1__6538400-6538487
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -26.26 kcal/mol |
AMFE -27.29 |
MFEI -0.72
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 1376 [download]
- Distribution of reads: random
((((((((.(((.(((((((((((((.((((((((((..(((((.(((........)))..))))).(((....)))...(((.((((((((((((..(((.(((((......((((..(((.....((((((((....)))))))))))...)))).....))))))))..)))))))).((((((...)))))).......(((..(((((....(((((.(((.(((((((((...))))))))).))).)))))....))))).)))((((((......((((((....))))))...))))))........(((((...))))).....((((.....))))))))))).)))))))))).)))))))..)))))).))).)))).))))
UGUUUGAACAAAUGUGGAUCCAUUAUUGGGCUCAUUGAUGACUAUUGGAUUAGGGCCCAUUUGGUUCGCCGUUUGGCACGCUACAGCCACAAAUGACACGUGUAGGAGAGGAAGACGGAGUUAACGGGGAUGGUCACGGGAUCAUCCAAUGAACGUCAGUUAUUUUAAUGACUCAUUUGUAUUUGGCCACGUCAAAAUAUUUAGCAAAAUUCAAAUAUUUUAACUUCUUUUUUGUUAUAAACAGAGAAAGAGAUAGAAGAAAUGAGUGUGUUAUUCCAUUGAGAUAAAGGGUUCUUUAUAUAGGAGUACAAGGAAAAGGGUAAAACCCUAGAAACAUCUAGAUGAUGGGCUUAGAUGAUGGGCCUUAUAAUGGGCAUCCAUCUUUAUUCAUAACA
((((((((.(((.(((((((((((((.((((((((((..............................................................................................................................................................................................................................................................................................................................)))))))))).)))))))..)))))).))).)))).))))
............................................................................................................................................................................................................................................************************....................................................................................................................................... length=24 depth=12
..................................................................................................................................................................................................................................................************************................................................................................................................................. length=24 depth=5
.....................................................................................................................................................................................................................................................************************.............................................................................................................................. length=24 depth=10
........................................................................................................................................................................................................................................................************************........................................................................................................................... length=24 depth=8
............................................................................................................................................................................................................................................................************************....................................................................................................................... length=24 depth=6
................................................................................................................................................................................................................................................................................************************................................................................................................... length=24 depth=8
..................................................................................................................................................................................................................................................................................***********************.................................................................................................. length=23 depth=5
......................................................................................................................................................................................................................................................................................................................................************************............................................. length=24 depth=11
......................................................................................................................................................................................................................................................................................................................................*************************............................................ length=25 depth=15
.......................................................................................................................................................................................................................................................................................................................................************************............................................ length=24 depth=66
........................................................................................................................................................................................................................................................................................................................................************************........................................... length=24 depth=28
.........................................................................................................................................................................................................................................................................................................................................***********************........................................... length=23 depth=16
.........................................................................................................................................................................................................................................................................................................................................************************.......................................... length=24 depth=5
..........................................................................................................................................................................................................................................................................................................................................***********************.......................................... length=23 depth=8
...........................................................................................................................................................................................................................................................................................................................................************************........................................ length=24 depth=15
...............................................................................................................................................................................................................................................................................................................................................************************.................................... length=24 depth=32
................................................................................................................................................................................................................................................................................................................................................************************................................... length=24 depth=5
.................................................................................................................................................................................................................................................................................................................................................************************.................................. length=24 depth=16
...................................................................................................................................................................................................................................................................................................................................................*******************..................................... length=19 depth=5
...................................................................................................................................................................................................................................................................................................................................................*********************................................... length=21 depth=10
...................................................................................................................................................................................................................................................................................................................................................***********************................................. length=23 depth=10
...................................................................................................................................................................................................................................................................................................................................................AGAUGAUGGGCUUAGAUGAUGGGC................................ length=24 depth=760
....................................................................................................................................................................................................................................................................................................................................................******************..................................... length=18 depth=5
....................................................................................................................................................................................................................................................................................................................................................**********************................................. length=22 depth=5
....................................................................................................................................................................................................................................................................................................................................................***********************................................ length=23 depth=27
....................................................................................................................................................................................................................................................................................................................................................************************............................... length=24 depth=17
.....................................................................................................................................................................................................................................................................................................................................................************************.............................. length=24 depth=12
.......................................................................................................................................................................................................................................................................................................................................................********************................................ length=20 depth=8
.......................................................................................................................................................................................................................................................................................................................................................*********************............................... length=21 depth=32
.......................................................................................................................................................................................................................................................................................................................................................***********************............................. length=23 depth=7
miRBase alignment: none
-
Chromosome: 1
-
Position: 6,592,984-6,593,131 (148 nt)
-
Strand: +
-
G+C content: 36.49 %
- miRNA sequence: AAAGAUAAAGCGGAUUAAACUGUU (24 nt)
- miRNA depth: 12 (weight: 12)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -34.20 kcal/mol |
AMFE -23.11 |
MFEI -0.63
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 22 [download]
- Distribution of reads: one island ★
GACGGACAAAUCUGUUUAUGUUUGUAGUGUUGAGGACUCAAAAAAAAAAAACUCAUUGCUGACCAACUACAAACCGCUUUUACAUACAAAUAGAGUUGCUCCGCAAUGGUUUGUUGUCUACCCUAAAGAUAAAGCGGAUUAAACUGUU
(((((...(((((((((.(((((.(((.((.((.(((...........((((.((((((.((.(((((.........................))))).)).))))))))))))).)).)).))).))))))))))))))...)))))
[[[[[...[[[[[[[[[.[[[[[.[ ].]]]]]]]]]]]]]]...]]]]]
............................................................................................................................AAAGAUAAAGCGGAUUAAACUGUU length=24 depth=12
.............................................................................................................................********************... length=20 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 50,512-50,681 (170 nt)
-
Strand: +
-
G+C content: 51.18 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -36.38 kcal/mol |
AMFE -45.29 |
MFEI -0.89
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 21 [download]
- Distribution of reads: random
(((((((.((((..((((((((((.(((.((((((..(((((.((((((.....((((((....))))))))))))((..(((.((((.(((((..((((....))))))))).)))).)))..)).)))))..)))))).)))))))))).)))..).))).)))))))
GGUGAGCUGGACAAGCUAGGAUGAACUAGCUAGCUAAGUUAGGUGAGCUAAACCAGCUGGAAGACUAGCUAGCUCAGCUAGGUGAGCUGACCGACAGCUCGACUGAGCUCGGUGAGCUGAUCGUGUACUGACCCAGCUGGGUGGUCAUUCUAAGUAAGAUCUUGCUUACU
(((((((.((((..((((((((((.(((.((((((..(((((.....................................................................................)))))..)))))).)))))))))).)))..).))).)))))))
.........................................................................................................************************......................................... length=24 depth=3
................................................................................................................**********************.................................... length=22 depth=2
.................................................................................................................************************................................. length=24 depth=4
miRBase alignment: none
-
Chromosome: 4
-
Position: 77,043-77,331 (289 nt)
-
Strand: -
-
G+C content: 46.37 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -59.19 kcal/mol |
AMFE -35.54 |
MFEI -0.77
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 213 [download]
- Distribution of reads: random
(((((.(((..((.((..((((((((((..(((((.(((((..(.((.(((.(((((.(((((......(((((.....((((((((.((((....((((((..((((........)))).))))))..((((((.......(((((((..((((.........)))).)))))))..))))))....(((((...)))))..))))...))))))))))))).....)))))))))).)))..)).)..))))))))))..))))))))))..)).)).))).)))))
UGGGCCUCUUCACCUAUUGCCAAUUGGUUUUAGGUUGGAAACCCACACUCUAAUAUGGUAUCAGAGCCUGAUCCGCACAUACUCAACCCGAUCCACAUCGGUCCGGCCCAAUACUUGGCCCGCCGAUCCAUGCCCAAACAUAUCGAGAUCAAUGGCUAGAAGAACCAUCAUCUCGAGGGGGCGUAUUAGGGACAAUGUCCCACAUCGAAAGUUGAGUAGGAUCUUAAGUGAUAUAUAUAAGAUAUGAGCCUUUCCAUCUAUUGCCAAUUGGUUUUAUGUUGGAAGCCCA
(((((.(((..((.((..((((((((((..(((((.(((((..(.((.(((.(((((.(((((......(((((.....((((((((.((((...............................................................................................................))))...))))))))))))).....)))))))))).)))..)).)..))))))))))..))))))))))..)).)).))).)))))
...............************************.......................................................................................................................................................................................................................................................... length=24 depth=17
............................................................................***********************.............................................................................................................................................................................................. length=23 depth=2
....................................................................................************************..................................................................................................................................................................................... length=24 depth=10
.....................................................................................................................................************************.................................................................................................................................... length=24 depth=6
.......................................................................................................................................***********************................................................................................................................................... length=23 depth=23
.......................................................................................................................................************************.................................................................................................................................. length=24 depth=9
........................................................................................................................................************************................................................................................................................................. length=24 depth=6
.........................................................................................................................................***********************................................................................................................................................. length=23 depth=3
.........................................................................................................................................************************................................................................................................................................ length=24 depth=4
...........................................................................................................................................*******************................................................................................................................................... length=19 depth=2
...........................................................................................................................................*********************................................................................................................................................. length=21 depth=3
...........................................................................................................................................**********************................................................................................................................................ length=22 depth=2
...........................................................................................................................................***********************............................................................................................................................... length=23 depth=2
...........................................................................................................................................************************.............................................................................................................................. length=24 depth=33
............................................................................................................................................***********************.............................................................................................................................. length=23 depth=2
.............................................................................................................................................************************............................................................................................................................ length=24 depth=8
.............................................................................................................................................*************************........................................................................................................................... length=25 depth=4
..............................................................................................................................................************************........................................................................................................................... length=24 depth=2
..............................................................................................................................................................................************************........................................................................................... length=24 depth=2
.................................................................................................................................................................................................************************........................................................................ length=24 depth=2
..................................................................................................................................................................................................................**********************......................................................... length=22 depth=2
..................................................................................................................................................................................................................***********************........................................................ length=23 depth=3
...................................................................................................................................................................................................................******************............................................................ length=18 depth=2
...................................................................................................................................................................................................................************************...................................................... length=24 depth=2
....................................................................................................................................................................................................................***********************...................................................... length=23 depth=9
......................................................................................................................................................................................................................*******************........................................................ length=19 depth=3
......................................................................................................................................................................................................................***********************.................................................... length=23 depth=3
.......................................................................................................................................................................................................................**********************.................................................... length=22 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 77,276-77,585 (310 nt)
-
Strand: +
-
G+C content: 45.16 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -43.29 kcal/mol |
AMFE -36.81 |
MFEI -0.82
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 103 [download]
- Distribution of reads: random
((((.(((.(((((((((((.(((((...((((((((...((.((...((((((((((...................(((((((((((((..(((((.((((((...)))))......((((.(((((((((((((((.....))))))).)))))))...)...))))..(.((((((.((((........)))).).))))).)).))))))))))))).....)))))...((((((((...))))))))))))))))))..)).))...))))))))...))))).).))))))))))))).))))
AUAUUAGAGUGUGGGUUUCCAACCUAAAACCAAUUGGCAAUAGGUGAAGAGGCCCAUAUCUUAUAUAUACCACUUAAGAUCUUAUUCAACUUCCGAUGUGGGAUAUUAUCCCUAAUACGCCCCCUCGAGAUGAUGGUUCUUCUAGCCAUUGAUCUCGAUAUGUUUGGGCAUGGAUCGGCGGGCCAAGUAUUGGGCCGGACCGAUGUGGAUCGGGUUGAGUAUGUGCGGAUCGGGGUCUGAUACCAUAUCAGACUGUGGGCUUCCAACGUAAAACUAAUUGGCAAUAGGUGGAGAGGCUCAUAUCUUAUAU
((((.(((.(((((((((((.(((((...((((((((...((.((...((((((((((...................................................................................................................................................................................................))))))))))..)).))...))))))))...))))).).))))))))))))).))))
<------miRBase------->
..............................************************................................................................................................................................................................................................................................................................ length=24 depth=4
.......................................................************************....................................................................................................................................................................................................................................... length=24 depth=5
.........................................................************************..................................................................................................................................................................................................................................... length=24 depth=3
..............................................................************************................................................................................................................................................................................................................................ length=24 depth=3
................................................................................................................************************.............................................................................................................................................................................. length=24 depth=3
................................................................................................................................................************************.............................................................................................................................................. length=24 depth=2
.................................................................................................................................................************************............................................................................................................................................. length=24 depth=2
......................................................................................................................................................************************........................................................................................................................................ length=24 depth=2
..............................................................................................................................................................******************...................................................................................................................................... length=18 depth=2
............................................................................................................................................................................................************************.................................................................................................. length=24 depth=9
................................................................................................................................................................................................................************************.............................................................................. length=24 depth=2
.........................................................................................................................................................................................................................................................******************........................................... length=18 depth=12
............................................................................................................................................................................................................................................................***********************................................... length=23 depth=3
.................................................................................................................................................................................................................................................................................************************............. length=24 depth=2
...................................................................................................................................................................................................................................................................................************************........... length=24 depth=2
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 20-41
U G - A AAA CAA G GAA UCUUAUAUAUACCACUUAAGAUCUUAUUCAACUUCCGAUGUGGGAUAUUAUCCCUAAUACGCCCCCUCGAGAUGAUGGUUCUUCUAGCCAUUGAUC
AUAU AGA UGUGGGUUUC C ACCUA CCAAUUGG UA GU GAGGCCCAUA U
|||| ||| |||||||||| | ||||| |||||||| || || |||||||||| C
UAUA UCU AUACUCGGAG G UGGAU GGUUAAUC AU CA CUUCGGGUGU G
U - A G AAC AAA G AC- CAGACUAUACCAUAGUCUGGGGCUAGGCGUGUAUGAGUUGGGCUAGGUGUAGCCAGGCCGGGUUAUGAACCGGGCGGCUAGGUACGGGUUUGUAUA
Alignments
query 20 CAACCUAAAACCAAUUGGCAAU 41
miRBase 1 CAACUUAAAAUCAGUUGGCAAU
**** ***** ** ********
miRBase 1 : MIMAT0017923 |
-
Chromosome: 4
-
Position: 297,901-298,116 (216 nt)
-
Strand: -
-
G+C content: 31.02 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -13.88 kcal/mol |
AMFE -24.54 |
MFEI -0.79
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 301 [download]
- Distribution of reads: random
((((..(((((((.(((((((((.((((..((((.((((...(((((.......((((((.((((.((((.......)))).)))))))))).....(((((((((((((...((((....)))).....)))))))))))))((((......)))).....)))))...))))........))))..)))).))))))))).)))))))..))))
CAUGUUUUAAAUGGUAGUUAACUCUUAGUUCAUGUUUAAAAUGGCCACAUAUAAAGUCGAUGUUUAAAAUGACCACAAUUUAAAACUUGACUAAUAAAGUCGUUAACGGAUGAUAACAGAAGUUAGAAGUUCCGUUAAUGACUGAUAACGGCAUGUCUAUUUUGGCCUGGUUAACGAAUAAUCAUGUUUUAAAAGUUAGUUAACGUUUAGUUCAUG
((((..(((((((.(((((((((.((((..((((....................................................................................................................................................))))..)))).))))))))).)))))))..))))
......................................************************.......................................................................................................................................................... length=24 depth=15
.......................................***********************.......................................................................................................................................................... length=23 depth=17
.......................................************************......................................................................................................................................................... length=24 depth=5
............................................................................................***********************..................................................................................................... length=23 depth=13
.........................................................................................................................************************....................................................................... length=24 depth=50
..........................................................................................................................************************...................................................................... length=24 depth=17
...........................................................................................................................***********************...................................................................... length=23 depth=6
...........................................................................................................................************************..................................................................... length=24 depth=15
............................................................................................................................*********************....................................................................... length=21 depth=7
............................................................................................................................**********************...................................................................... length=22 depth=4
............................................................................................................................***********************..................................................................... length=23 depth=28
............................................................................................................................************************.................................................................... length=24 depth=4
.............................................................................................................................***********************.................................................................... length=23 depth=3
.............................................................................................................................************************................................................................... length=24 depth=7
...............................................................................................................................***********************.................................................................. length=23 depth=3
..............................................................................................................................................************************.................................................. length=24 depth=7
...............................................................................................................................................************************................................................. length=24 depth=3
................................................................................................................................................************************................................................ length=24 depth=18
...............................................................................................................................................................************************................................. length=24 depth=4
miRBase alignment: none
-
Chromosome: 4
-
Position: 298,193-298,561 (369 nt)
-
Strand: -
-
G+C content: 30.89 %
- miRNA sequence: AAGCAGAACCCAAGAACAUAGUUU (24 nt)
- miRNA depth: 176 (weight: 176)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -50.55 kcal/mol |
AMFE -23.93 |
MFEI -0.77
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 300 [download]
- Distribution of reads: random
((((((((((.(((...(((((((((..((....(((((((((((((.((((((.((...(((((((((...((((((((((.((((((((..(((((............)))))....))))))))....(((((((........((((.((((((((........)))))))).))))..(((((((((..(((((....)))))....)))))))))......((((..(((((((.....))))))).)))))))))))....................))))))))))...))))).))))...)).)))))).))))))))))))).....))..))))..)))))....)))))))))))))
GUUAAUGACUGCUAAUCUAUCCAAAACAACGUCGUUUGAGAAUAAUCGAAAGAACAUAAAGAUUUUUUCCAUAUGAAAUUUCCAUAUCAAAAAGUUUUAAGCAGAACCCAAGAACAUAGUUUGAUAUAGAGAUAACCGCAGUGCCAGUAAUUGGAAGCAAAUAAAGGUGUUUUCGUUUAUAUAUUUGUUUUAAAUUUUAAAAGGAAUCAUGGAAGCAAAUUGAAGACUCAUUAUAAGAUUGUUCGUCUUGUUUGAGCGGUUGUAGAAAUAGAUUUGGGGAACUGGAGUUUCAUUUAGAAAACAAUCCUGAUUUUCUUUGGAUUAUUCUCAAAACAAAGUCAUUUUAGGGAUACUAAUAGAGUCGUUAAC
((((((((((.(((...(((((((((..((....(((((((((((((.((((((.((...(((((((((...((((((((((.........................................................................................................................................................................................................))))))))))...))))).))))...)).)))))).))))))))))))).....))..))))..)))))....)))))))))))))
.........................************************................................................................................................................................................................................................................................................................................................................................ length=24 depth=11
..........................***********************................................................................................................................................................................................................................................................................................................................................ length=23 depth=9
................................................................................................************************......................................................................................................................................................................................................................................................... length=24 depth=3
..................................................................................................AAGCAGAACCCAAGAACAUAGUUU....................................................................................................................................................................................................................................................... length=24 depth=176
...................................................................................................***********************....................................................................................................................................................................................................................................................... length=23 depth=3
...................................................................................................************************...................................................................................................................................................................................................................................................... length=24 depth=6
....................................................................................................************************..................................................................................................................................................................................................................................................... length=24 depth=3
...............................................................................................................***********************........................................................................................................................................................................................................................................... length=23 depth=3
................................................................................................................................................................................................................************************......................................................................................................................................... length=24 depth=22
.......................................................................................................................................................................................................................************************.................................................................................................................................. length=24 depth=3
.......................................................................................................................................................................................................................................................***********************................................................................................................... length=23 depth=3
.............................................................................................................................................................................................................................................................************************............................................................................................ length=24 depth=6
.........................................................................................................................................................................................................................................................................................................................................................************************ length=24 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 313,266-313,379 (114 nt)
-
Strand: -
-
G+C content: 29.82 %
- miRNA sequence: CUUGGUAUAUUCAAAUUAGGAUU (23 nt)
- miRNA depth: 11 (weight: 11)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -23.80 kcal/mol |
AMFE -20.88 |
MFEI -0.7
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 36 [download]
- Distribution of reads: random
CUAUCAAAAACUUAAAACCUUUCAAAACCUCUUGGUAUAUUCAAAUUAGGAUUCUUCAUUCAUGAAAUCAUUAUUCCAAGGGUUUUGGGAUGUCUUACGAAAUAAUUUUGGUAG
(((((((((.(.(((.((.((((((((((.(((((.(((......(((.((........)).)))......))).))))))))))))))).)).))).)......)))))))))
...............************************........................................................................... length=24 depth=2
.............................************************............................................................. length=24 depth=2
..............................CUUGGUAUAUUCAAAUUAGGAUU............................................................. length=23 depth=11
...........................................................................********************................... length=20 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 434,304-434,483 (180 nt)
-
Strand: +
-
G+C content: 31.11 %
- miRNA sequence: AGUGACCAUUGGAGCAUCGUGCCU (24 nt)
- miRNA depth: 72 (weight: 72)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -66.37 kcal/mol |
AMFE -40.94 |
MFEI -1.32
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 152 [download]
- Distribution of reads: two islands ★★
(((((((((((((.(((((((((((((((((((...(((((((((.(((((((.(((((((.((((((...))))))..(((((.((((...........)))).))))).....))))))).))))))).)))))))))...)))))))))))))))....)))).)))))))))))))
UGACUCUAUUUUAGAGGCUGCUCCAAUGGUCACAUGUAAAAUAGAAUUUUCUUUAAAAUAGAGGAGAUAUCAUCUCUAUAAAUAAAGAUCUCUACAUUUAGUCUCUAUUUUUUUCCUAUUUUUGAGGAAACUCUAUUUUAAAAGUGACCAUUGGAGCAUCGUGCCUAUAAAAUAGGGUCA
(((((((((((((.(((((((((((((((((((...(((((((((.(((((((.(((((((......................................................))))))).))))))).)))))))))...)))))))))))))))....)))).)))))))))))))
[[[[[[[[[[[[[[[[[[[. .]]]]]]]]]]]]]]]....]]]]
<-----miRBase----->
............************************................................................................................................................................................ length=24 depth=2
.......................************************..................................................................................................................................... length=24 depth=3
........................************************.................................................................................................................................... length=24 depth=3
............................************************................................................................................................................................ length=24 depth=2
................................................................................************************............................................................................ length=24 depth=2
.....................................................................................................................................************************....................... length=24 depth=2
......................................................................................................................................***********************....................... length=23 depth=2
........................................................................................................................................************************.................... length=24 depth=4
...........................................................................................................................................******************....................... length=18 depth=2
.............................................................................................................................................************************............... length=24 depth=3
..............................................................................................................................................AGUGACCAUUGGAGCAUCGUGCCU.............. length=24 depth=72
..............................................................................................................................................*************************............. length=25 depth=2
...............................................................................................................................................***********************.............. length=23 depth=4
................................................................................................................................................***********************............. length=23 depth=13
.................................................................................................................................................*********************.............. length=21 depth=4
.................................................................................................................................................**********************............. length=22 depth=3
.................................................................................................................................................************************........... length=24 depth=4
...................................................................................................................................................************************......... length=24 depth=2
.........................................................................................................................................................************************... length=24 depth=5
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 47-65
G ---- AUG A U AGGAGAUAUCAUCUCUAUAAAUAAAG
UGACUCUAUUUUA AGGC UGCUCCAAUGGUCAC UAAAAUAGA UUUUCUU AAAAUAG A
||||||||||||| |||| ||||||||||||||| ||||||||| ||||||| |||||||
ACUGGGAUAAAAU UCCG ACGAGGUUACCAGUG AUUUUAUCU AAAGGAG UUUUAUC U
A UGCU AAA C U CUUUUUUUAUCUCUGAUUUACAUCUC
Alignments
query 47 UUUUCU-UUAA-AAUAGAGGA 65
miRBase 1 UUUUCUCUUAACAACAGAGGA
****** **** ** ******
miRBase 1 : MIMAT0033872 |
-
Chromosome: 4
-
Position: 618,259-618,526 (268 nt)
-
Strand: -
-
G+C content: 15.67 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -12.02 kcal/mol |
AMFE -19.74 |
MFEI -1.26
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 119 [download]
- Distribution of reads: random
(((((((((((.((((((.(((((...((..((((........(((((((..((((((((((((((.(((..........))).)))))))))))))).((((......)))).(((((((((((((((((((((((((...)))))))))))))))))))))))))(((((((((...(((((................))))))))))))))........))))))).......))))..))))))))))))).)).)))))))))
AAAAAAUUGCAAGAUUAUCAUAUAAGUCACUCAACCAUAUACAGUAGAAACUCUAUAAAUUAAUAAUGUUGGGACCAUGAAAUUUUAUUAAUUUAUAGUGAUAUUAAUUUAUCCUAUAAAUUAAUAUUUAUUAAUUUAUCGUAUAAGUUAAUAAAUAUUAAUUUAUAAAAAUAUUUAUGUAAAAUACAAUACUAUAUUCUUUUUAAAAUGUUUUUAAUUAAUUUUCUAUAUGCUUUGUUGUUUGUAUAUAUAAUUAUGUUAGUUUUUU
(((((((((((.((((((.(((((...((..((((........(((((((............................................................................................................................................................................))))))).......))))..))))))))))))).)).)))))))))
................................************************.................................................................................................................................................................................................................... length=24 depth=2
..................................***********************................................................................................................................................................................................................................... length=23 depth=5
........................................************************............................................................................................................................................................................................................ length=24 depth=2
..........................................***********************........................................................................................................................................................................................................... length=23 depth=4
...................................................************************................................................................................................................................................................................................. length=24 depth=2
....................................................************************................................................................................................................................................................................................ length=24 depth=30
.....................................................***********************................................................................................................................................................................................................ length=23 depth=15
.....................................................************************............................................................................................................................................................................................... length=24 depth=4
......................................................**********************................................................................................................................................................................................................ length=22 depth=4
......................................................***********************............................................................................................................................................................................................... length=23 depth=8
......................................................************************.............................................................................................................................................................................................. length=24 depth=5
.......................................................*********************................................................................................................................................................................................................ length=21 depth=5
........................................................*********************............................................................................................................................................................................................... length=21 depth=3
.............................................................***********************........................................................................................................................................................................................ length=23 depth=2
................................................................************************.................................................................................................................................................................................... length=24 depth=7
...................................................................***********************.................................................................................................................................................................................. length=23 depth=3
miRBase alignment: none
-
Chromosome: 4
-
Position: 743,281-743,392 (112 nt)
-
Strand: -
-
G+C content: 22.32 %
- miRNA sequence: AUUUAGGGAAUCUAGUUUUUACUC (24 nt)
- miRNA depth: 19 (weight: 19)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -19.50 kcal/mol |
AMFE -17.41 |
MFEI -0.78
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 25 [download]
- Distribution of reads: one island ★
GAUUUUAAAAUUUUGGUUUCCCUACAAUUUGAAAAACUAUUUUGUUGUAAUUUUCCAUUCAUGAUUUUAGUUUUUUAGUUUUUCUAAAAUUUAGGGAAUCUAGUUUUUACUC
((...(((((..((((.(((((((.((((((((((((((.....(((.((((..........)))).))).....)))))))))..)))))))))))).)))).))))).))
[[...[[[[[..[[[[.[[[[[[[.[[[ ]]]]]]]]]].]]]].]]]]].]]
........................................................................................AUUUAGGGAAUCUAGUUUUUACUC length=24 depth=19
............................................................................................******************** length=20 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 743,386-743,470 (85 nt)
-
Strand: -
-
G+C content: 22.35 %
- miRNA sequence: AAGGUCUUGAAUGUUUUGUAGAUU (24 nt)
- miRNA depth: 31 (weight: 31)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -24.00 kcal/mol |
AMFE -28.24 |
MFEI -1.26
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 44 [download]
- Distribution of reads: one island ★
UAAGGUCUUGAAUGUUUUGUAGAUUUUGUAAGAGUUUUUGGAUUUUGUUUUUCAAAAACUUAUUUUCAUAACAAUCAAGAUUUUA
(((((((((((.((((.((.((((.......(((((((((((........))))))))))))))).)).)))).)))))))))))
[[[[[[[[[[.[[[[.[[.[[[[. ]]]]].]].]]]].]]]]]]]]]]
.AAGGUCUUGAAUGUUUUGUAGAUU............................................................ length=24 depth=31
..***********************............................................................ length=23 depth=2
....................************************......................................... length=24 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 983,183-983,345 (163 nt)
-
Strand: -
-
G+C content: 23.31 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -20.14 kcal/mol |
AMFE -18.77 |
MFEI -0.81
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 247 [download]
- Distribution of reads: random
(((((.((((((((..((((...((((.(((((...........................(((((.((((((((........)))))))).)))))((((((...))))))..((((((....)))))).)))))..))))....)))).)))))))))))))
UCAAGUGAGAAACUAUGAGAUACUCUCUACAAGCCAUCAUUACAUUUCUCUUCUUUUAAUUGAUUCAAUUUUUACACUAAAAUAAAAAUUUAAUCAUAUAAUAAAAUUAUAAUAAAAUACUAAUAUUUUUCUUGUUCGAGAAACUUUUUGGGUUUCUCCUUGA
(((((.((((((((..((((...((((.(((((.................................................................................................)))))..))))....)))).)))))))))))))
<----miRBase---->
<----miRBase---->
************************........................................................................................................................................... length=24 depth=5
.***********************........................................................................................................................................... length=23 depth=5
..**********************........................................................................................................................................... length=22 depth=8
..***********************.......................................................................................................................................... length=23 depth=9
..************************......................................................................................................................................... length=24 depth=65
....************************....................................................................................................................................... length=24 depth=26
......***********************...................................................................................................................................... length=23 depth=3
......************************..................................................................................................................................... length=24 depth=3
.......*********************....................................................................................................................................... length=21 depth=6
.......************************.................................................................................................................................... length=24 depth=31
.........************************.................................................................................................................................. length=24 depth=4
..........************************................................................................................................................................. length=24 depth=13
...........************************................................................................................................................................ length=24 depth=17
..............************************............................................................................................................................. length=24 depth=3
...................************************........................................................................................................................ length=24 depth=3
..............................************************............................................................................................................. length=24 depth=3
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 98-114
U AU UAC- U- CCAUCAUUACAUUUCUCUUCUUUUAAUUGAUUCAAUUUUUACACUAA
UCAAG GAGAAACU GAGA UCUC ACAAG A
||||| |||||||| |||| |||| ||||| A
AGUUC CUCUUUGG UUUU AGAG UGUUC U
- G- UCAA CU UUUUUAUAAUCAUAAAAUAAUAUUAAAAUAAUAUACUAAUUUAAAAA
Alignments
query 98 AUAAUAAA-AUUAUAAU-A 114
miRBase 1 AUAAUAAAAAUAAUAAUGA
******** ** ***** *
miRBase 1 : MIMAT0007396 |
Prediction : 109-125
U AU UAC- U- CCAUCAUUACAUUUCUCUUCUUUUAAUUGAUUCAAUUUUUACACUAA
UCAAG GAGAAACU GAGA UCUC ACAAG A
||||| |||||||| |||| |||| ||||| A
AGUUC CUCUUUGG UUUU AGAG UGUUC U
- G- UCAA CU UUUUUAUAAUCAUAAAAUAAUAUUAAAAUAAUAUACUAAUUUAAAAA
Alignments
query 109 AUAAUAAA-AUACUAAU-A 125
miRBase 1 AUAAUAAAAAUAAUAAUGA
******** *** **** *
miRBase 1 : MIMAT0007396 |
-
Chromosome: 4
-
Position: 983,292-983,369 (78 nt)
-
Strand: +
-
G+C content: 35.90 %
- miRNA sequence: AUGUAAUGAUGGCUUGUAGAGAGU (24 nt)
- miRNA depth: 279 (weight: 279)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -25.80 kcal/mol |
AMFE -33.08 |
MFEI -0.92
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 567 [download]
- Distribution of reads: one island ★
AGAAGAGAAAUGUAAUGAUGGCUUGUAGAGAGUAUCUCAUAGUUUCUCACUUGAGAAACUUUCUACACUCUCUCUUCU
((((((((............(..((((((((((.(((((.(((.....)))))))).))))))))))..)))))))))
...........[..[[[[[[[[[[ ]]]]]]]]]]..]
.........***********************.............................................. length=23 depth=15
.........AUGUAAUGAUGGCUUGUAGAGAGU............................................. length=24 depth=279
..........***********************............................................. length=23 depth=10
..........************************............................................ length=24 depth=21
...........***********************............................................ length=23 depth=14
............*********************............................................. length=21 depth=17
.............********************............................................. length=20 depth=15
.............*********************............................................ length=21 depth=67
.............************************......................................... length=24 depth=14
miRBase alignment: none
-
Chromosome: 4
-
Position: 985,565-985,737 (173 nt)
-
Strand: +
-
G+C content: 36.42 %
- miRNA sequence: GAGAGAGAGAGAGAGAGAGAGAG (23 nt)
- miRNA depth: 4 (weight: 0.12)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -24.93 kcal/mol |
AMFE -27.63 |
MFEI -0.76
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 10 [download]
- Distribution of reads: one island ★
((((.((.((((((((.(((.((.(((((((((((((((((........(((...((((((((((..........)))))))).))..)))..((((((((.(((...)))))))))))..))))))))...........))))))))).)).))).)))))))).)).))))
UUCUGUUCUUCUCUCUGUUUGUUUGAAUUCAGUAGCAAUUGUGUAUUGCGUCUUAACGUCUUAAGCUAAUAUACCCUUAAGACUGUGUGAUUUUUCUUAGUUAUGAGUCGUAUUAAGAAUCCAAUUGCUUCGUUUUCUGAAUUGGAUUCCGAGAGAGAGAGAGAGAGAGAGAG
((((.((.((((((((.(((.((.(((((((((...........................................................................................................))))))))).)).))).)))))))).)).))))
[[[[.[[.[[[[[[[[.[[[.[[ ]].]]].]]]]]]]].]].]]]]
......................................................................................................................................................**********************. length=22 depth=3
......................................................................................................................................................GAGAGAGAGAGAGAGAGAGAGAG length=23 depth=4
.......................................................................................................................................................********************** length=22 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,001,115-1,001,209 (95 nt)
-
Strand: +
-
G+C content: 15.79 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -12.50 kcal/mol |
AMFE -13.16 |
MFEI -0.83
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 164 [download]
- Distribution of reads: random
GUAAAGAUGAUUUAAAAAUAUAUGAUAUUAUUUUAUUUGAUUUUAAAUGUAUAGUAAACUGUGAGUUGUAUAUGUUUUAUUGAUAUUAUCUAUAU
(((.((((((((((((((((((((...((((((((((..((.......))..))))))..))))....)))))))))..)))).))))))).)))
...........................................************************............................ length=24 depth=6
............................................***********************............................ length=23 depth=3
.............................................************************.......................... length=24 depth=22
..............................................************************......................... length=24 depth=7
.................................................***********************....................... length=23 depth=21
..................................................**********************....................... length=22 depth=18
..................................................************************..................... length=24 depth=14
....................................................************************................... length=24 depth=37
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,023,847-1,024,244 (398 nt)
-
Strand: -
-
G+C content: 34.17 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -110.57 kcal/mol |
AMFE -54.27 |
MFEI -1.59
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 364 [download]
- Distribution of reads: random
((((((.(((((.(((((((((((((((((((.(((((((((((((((((((((((((((.(((((((((((((((((((((((((((((.((((((((((........)))))).)))).(((((((((((.(.(((((((.(((((((((((((((((.(((((((((.(((((((((((.((.............((((((((.(((...(((........)))...))).))))))))..))))))))))))).))))))))).))))))))))))))))).))))))).).))))))))))).))))))))))))))))))))))))))))).))))))))))))))))))))))))))).))))))))))))))))))).))))).))))))
AACUUUCAAAAAAUGGCCAAAUUAACCGUGAACUUUUGAAAUGGUCGUUUUAUACCUCAAAAAAAAGUUGAUUUCUAAUUUAACCUAUAAUUUAUUGUUGACUUCUAAUUUAACAUAUAAUUUAUCGUUGACCCAGCCAAAUAGACUCACCGUUAACACUCCUUAACAGCGCUCCUAACAGCCGUUACAAAACAUGGCCUAACAGCCGUUAUCGUUGGUUUAAGGACAAAAACAGUUGUUAGUUACGCUGUUAGGAGAGCUGUUAAGAAGUGUUAAUGGUGAGUCGAUUUGGCCGUGUCAACGAUAACUUAUAGGUUAAAUUAGAAGUCAACUUUUUGUUGAGGUAUAAAACGGCCAUUUCAAAAAUUCAUGGUUAAUUUGGCCACUUUUUAAAAGUU
((((((.(((((.(((((((((((((((((((.(((((((((((((((((((((((((((.(((((((((((((((((((((((((((((..........................................................................................................................................................................................................................))))))))))))))))))))))))))))).))))))))))))))))))))))))))).))))))))))))))))))).))))).))))))
<------miRBase------>
..................************************.................................................................................................................................................................................................................................................................................................................................................................... length=24 depth=37
...................**********************..................................................................................................................................................................................................................................................................................................................................................................... length=22 depth=2
...................***********************.................................................................................................................................................................................................................................................................................................................................................................... length=23 depth=13
...................************************................................................................................................................................................................................................................................................................................................................................................................... length=24 depth=2
....................*********************..................................................................................................................................................................................................................................................................................................................................................................... length=21 depth=5
.....................*********************.................................................................................................................................................................................................................................................................................................................................................................... length=21 depth=2
.......................************************............................................................................................................................................................................................................................................................................................................................................................... length=24 depth=33
..................................................................................................................************************.................................................................................................................................................................................................................................................................... length=24 depth=3
...........................................................................................................................************************........................................................................................................................................................................................................................................................... length=24 depth=23
...................................................................................................................................***********************.................................................................................................................................................................................................................................................... length=23 depth=2
........................................................................................................................................************************.............................................................................................................................................................................................................................................. length=24 depth=4
.........................................................................................................................................***********************.............................................................................................................................................................................................................................................. length=23 depth=4
...............................................................................................................................................************************....................................................................................................................................................................................................................................... length=24 depth=2
...........................................................................................................................................................************************........................................................................................................................................................................................................................... length=24 depth=3
..................................................................................................................................................................*********************....................................................................................................................................................................................................................... length=21 depth=2
.....................................................................................................................................................................................................***********************.................................................................................................................................................................................. length=23 depth=3
........................................................................................................................................................................................................************************.............................................................................................................................................................................. length=24 depth=3
.........................................................................................................................................................................................................************************............................................................................................................................................................................. length=24 depth=4
.........................................................................................................................................................................................................................................************************............................................................................................................................................. length=24 depth=3
............................................................................................................................................................................................................................................************************.......................................................................................................................................... length=24 depth=2
...............................................................................................................................................................................................................................................************************....................................................................................................................................... length=24 depth=2
................................................................................................................................................................................................................................................************************...................................................................................................................................... length=24 depth=9
..................................................................................................................................................................................................................................................***********************..................................................................................................................................... length=23 depth=6
...........................................................................................................................................................................................................................................................************************........................................................................................................................... length=24 depth=2
............................................................................................................................................................................................................................................................***********************........................................................................................................................... length=23 depth=2
............................................................................................................................................................................................................................................................************************.......................................................................................................................... length=24 depth=14
........................................................................................................................................................................................................................................................................************************.............................................................................................................. length=24 depth=5
.........................................................................................................................................................................................................................................................................************************............................................................................................................. length=24 depth=2
.............................................................................................................................................................................................................................................................................************************......................................................................................................... length=24 depth=2
...............................................................................................................................................................................................................................................................................***********************........................................................................................................ length=23 depth=3
...............................................................................................................................................................................................................................................................................************************....................................................................................................... length=24 depth=2
.................................................................................................................................................................................................................................................................................................................************************..................................................................... length=24 depth=3
.....................................................................................................................................................................................................................................................................................................................*********************.................................................................... length=21 depth=2
.....................................................................................................................................................................................................................................................................................................................************************................................................................. length=24 depth=9
......................................................................................................................................................................................................................................................................................................................***********************................................................................. length=23 depth=2
......................................................................................................................................................................................................................................................................................................................************************................................................................ length=24 depth=37
.........................................................................................................................................................................................................................................................................................................................................************************............................................. length=24 depth=2
............................................................................................................................................................................................................................................................................................................................................************************.......................................... length=24 depth=2
.........................................................................................................................................................................................................................................................................................................................................................************************............................. length=24 depth=6
............................................................................................................................................................................................................................................................................................................................................................*********************............................. length=21 depth=2
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 261-281
C A C A UUUAUUGUUGACUUCUAAUUUAACAUAUAAUUUAUCGUUGACCCAGCCAAAUAGACUCACCGUUAACACUCCUUAACAGCGCUCCUAACAGCCGUUACAAAACAUGGC
AACUUU AAAAA UGGCCAAAUUAACCGUGAA UUUUGAAAUGGUCGUUUUAUACCUCAA AAAAAGUUGAUUUCUAAUUUAACCUAUAA C
|||||| ||||| ||||||||||||||||||| ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
UUGAAA UUUUU ACCGGUUUAAUUGGUACUU AAAACUUUACCGGCAAAAUAUGGAGUU UUUUUCAACUGAAGAUUAAAUUGGAUAUU U
A C A G CAAUAGCAACUGUGCCGGUUUAGCUGAGUGGUAAUUGUGAAGAAUUGUCGAGAGGAUUGUCGCAUUGAUUGUUGACAAAAACAGGAAUUUGGUUGCUAUUGCCGACAA
Alignments
query 261 UGUUAAGAAGUGUUAAUGGUG 281
miRBase 1 UGUUAAGGAGUGUUAACGGUG
******* ******** ****
miRBase 1 : MIMAT0022395 | ath-miR5635d | ath-miR5635b | ath-miR5635c |
-
Chromosome: 4
-
Position: 1,116,570-1,116,885 (316 nt)
-
Strand: -
-
G+C content: 28.48 %
- miRNA sequence: ACGAAUCAAACAGUGAAAAGUGAC (24 nt)
- miRNA depth: 604 (weight: 302)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -152.00 kcal/mol |
AMFE -48.10 |
MFEI -1.69
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 1438 [download]
- Distribution of reads: one island ★
UUUAAGGGUUAAGUUGUAUACUAGUCACUUUUUAAAAUUUAAUUGAAAAAAUAGUCACUUUACUAUUUAUAAUUUGCAAAAUAGUCACUUUUCACUGUUUGAUUCGUACACUUAGUUUGUACACCACUUUUAGUGAUCAGAUGUAUAUAAAAAAUACACAGCGUACACUUUACAUUUGUACAAUAAAAGUGAUGUACAAUCCAAGUGUACGAAUCAAACAGUGAAAAGUGACUACUUUGCAAAUUAUGAAUAGUAAAGUGACUACUUUCCUAAUUAAGUUUGAAAAAGUGACUAUUUUGCCAAAACACCCCUUGAA
((((((((.....(((((...((((((((((((.(((((((((((..(((.(((((((((((((((((((((((((((((.((((((((((((((((((((((((((((((((.(.(((((((.((((((((.((..((((((((.........................))))))))..)).)))))))).))))))).).)))))))))))))))))))))))))))))))).))))))))))))))))))))))))))))).)))..))))))))))).))))))))))))...)).))).....))))))))
[[[[[[[[[[[[[[[[[[[[[[[[ ]]]]]]]]]]]]]]]]]]]]]]]]
<-----miRBase------> <-----miRBase----->
...........................................................................************************......................................................................................................................................................................................................................... length=24 depth=11
............................................................................***********************......................................................................................................................................................................................................................... length=23 depth=9
................................................................................************************.................................................................................................................................................................................................................... length=24 depth=48
.................................................................................***********************.................................................................................................................................................................................................................... length=23 depth=18
...................................................................................************************................................................................................................................................................................................................................. length=24 depth=13
.....................................................................................***********************................................................................................................................................................................................................................ length=23 depth=7
........................................................................................************************............................................................................................................................................................................................................ length=24 depth=23
..........................................................................................***********************........................................................................................................................................................................................................... length=23 depth=6
................................................................................................************************.................................................................................................................................................................................................... length=24 depth=32
...........................................................................................................................................................................************************......................................................................................................................... length=24 depth=6
.....................................................................................................................................................................................................***********************................................................................................................ length=23 depth=23
......................................................................................................................................................................................................************************.............................................................................................. length=24 depth=11
.............................................................................................................................................................................................................************************....................................................................................... length=24 depth=25
..............................................................................................................................................................................................................***********************....................................................................................... length=23 depth=6
..............................................................................................................................................................................................................************************...................................................................................... length=24 depth=12
................................................................................................................................................................................................................*********************....................................................................................... length=21 depth=7
................................................................................................................................................................................................................ACGAAUCAAACAGUGAAAAGUGAC.................................................................................... length=24 depth=604
................................................................................................................................................................................................................*************************................................................................................... length=25 depth=6
.................................................................................................................................................................................................................***********************.................................................................................... length=23 depth=18
.................................................................................................................................................................................................................************************................................................................................... length=24 depth=20
..................................................................................................................................................................................................................**********************.................................................................................... length=22 depth=8
..................................................................................................................................................................................................................***********************................................................................................... length=23 depth=29
...................................................................................................................................................................................................................*********************.................................................................................... length=21 depth=6
...................................................................................................................................................................................................................**********************................................................................................... length=22 depth=7
...................................................................................................................................................................................................................************************................................................................................. length=24 depth=28
....................................................................................................................................................................................................................************************................................................................................ length=24 depth=18
...............................................................................................................................................................................................................................................************************..................................................... length=24 depth=8
................................................................................................................................................................................................................................................***********************..................................................... length=23 depth=48
................................................................................................................................................................................................................................................************************.................................................... length=24 depth=14
.................................................................................................................................................................................................................................................**********************..................................................... length=22 depth=7
.................................................................................................................................................................................................................................................************************................................................... length=24 depth=6
..................................................................................................................................................................................................................................................************************.................................................. length=24 depth=47
............................................................................................................................................................................................................................................................................************************........................ length=24 depth=20
.............................................................................................................................................................................................................................................................................************************....................... length=24 depth=16
..............................................................................................................................................................................................................................................................................***********************....................... length=23 depth=8
...............................................................................................................................................................................................................................................................................************************..................... length=24 depth=13
miRBase alignment: ✓✓ presence of alignments that cover the miRNA locus (see reads cloud above)
Prediction : 70-89
UUAAG - UAC A AA A A A U C G AU UAUAAAAAAUA
UUUAAGGG UUG UA UAGUCACUUUUU AAAUUUAAUUG AAA UAGUCACUUUACUAUUUAUAAUUUGCAAA UAGUCACUUUUCACUGUUUGAUUCGUACACUU G UUGUACA CACUUUUA UG CAGAUGUA C
|||||||| ||| || |||||||||||| ||||||||||| ||| ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| | ||||||| |||||||| || |||||||| A
AAGUUCCC AAC GU AUCAGUGAAAAA UUUGAAUUAAU UUU AUCAGUGAAAUGAUAAGUAUUAAACGUUU AUCAGUGAAAAGUGACAAACUAAGCAUGUGAA C AACAUGU GUGAAAAU AC GUUUACAU C
CACAA C UUU G CC C C C U A A AU UUCACAUGCGA
Alignments
query 70 UAA-U-UUGCAAAAUAGUCACU 89
miRBase 1 UAAGUGUUGCAAAAUAGUCAUU
*** * ************** *
miRBase 1 : MIMAT0007378 |
Prediction : 108-126
UUAAG - UAC A AA A A A U C G AU UAUAAAAAAUA
UUUAAGGG UUG UA UAGUCACUUUUU AAAUUUAAUUG AAA UAGUCACUUUACUAUUUAUAAUUUGCAAA UAGUCACUUUUCACUGUUUGAUUCGUACACUU G UUGUACA CACUUUUA UG CAGAUGUA C
|||||||| ||| || |||||||||||| ||||||||||| ||| ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| | ||||||| |||||||| || |||||||| A
AAGUUCCC AAC GU AUCAGUGAAAAA UUUGAAUUAAU UUU AUCAGUGAAAUGAUAAGUAUUAAACGUUU AUCAGUGAAAAGUGACAAACUAAGCAUGUGAA C AACAUGU GUGAAAAU AC GUUUACAU C
CACAA C UUU G CC C C C U A A AU UUCACAUGCGA
Alignments
query 108 ACACUUAG-UUUGUACACCA- 126
miRBase 1 ACACUUAGUUUUGUACAACAU
******** ******** **
miRBase 1 : MIMAT0021047 |
-
Chromosome: 4
-
Position: 1,190,061-1,190,301 (241 nt)
-
Strand: -
-
G+C content: 24.07 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -35.00 kcal/mol |
AMFE -23.69 |
MFEI -0.98
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 101 [download]
- Distribution of reads: random
(((((((((.((((((..(((((((((((((((...............((((((((.(((((......))))).))....))))))..........(((((((.((((....))))...))))))).(((((..((((((.(.....).))))))...)))))...(((((.(((....((((((....))))))....))).))))))))))))))))))))..)))))).)))))))))
UAUUCCCUCCGUCCCACUAAGAUGAAUUUUUUAAAAAUUUCACAUUGUUUAAGAAAUAUUGUAUAAAGACAAUUUUAACCUCUUAAUAUACUUUAAAAAUAAUAAUAAAUAUUUAUCUAAUUAUUUAGUAAGGAUAAAACGGGAAAACAGUUUUAAAAUUUGCAUUGGAAAUAUAAAACUUCUAUCUUUGUGGGACAAAUAUUUUUCCUAGAAAAUUUAUCUUUAUGGGACAGAGGGAGUA
(((((((((.((((((..(((((((((((((((...............................................................................................................................................................................)))))))))))))))..)))))).)))))))))
<------miRBase------>
.***********************......................................................................................................................................................................................................................... length=23 depth=2
.************************........................................................................................................................................................................................................................ length=24 depth=3
............................................................................................................................********************................................................................................................. length=20 depth=2
............................................................................................................................************************............................................................................................. length=24 depth=2
.................................................................................................................................************************........................................................................................ length=24 depth=7
............................................................................................................................................................************************............................................................. length=24 depth=2
.............................................................................................................................................................************************............................................................ length=24 depth=8
..............................................................................................................................................................***********************............................................................ length=23 depth=6
...................................................................................................................................................................************************...................................................... length=24 depth=11
............................................................................................................................................................................***********************.............................................. length=23 depth=2
............................................................................................................................................................................************************............................................. length=24 depth=7
..............................................................................................................................................................................***********************............................................ length=23 depth=7
...........................................................................................................................................................................................*********************................................. length=21 depth=3
miRBase alignment: ✓ presence of alignments, which do not overlap the miRNA locus (see reads cloud above)
Prediction : 221-241
C CU AAAAUUUCACAUUGUUUAAGAAAUAUUGUAUAAAGACAAUUUUAACCUCUUAAUAUACUUUAAAAAUAAUAAUAAAUAUUUAUCUA
UAUUCCCUC GUCCCA AAGAUGAAUUUUUUA A
||||||||| |||||| ||||||||||||||| U
AUGAGGGAG CAGGGU UUCUAUUUAAAAGAU U
A AU CCUUUUUAUAAACAGGGUGUUUCUAUCUUCAAAAUAUAAAGGUUACGUUUAAAAUUUUGACAAAAGGGCAAAAUAGGAAUGAUUUA
Alignments
query 221 -CUUUAUGGGACAGAGGGAGU- 241
miRBase 1 ACAUUAUGGGACGGAGGGAGU-
miRBase 2 --UUUAUGGAACGGAGGGAGU-
****** ** *********
miRBase 1 : MIMAT0005987 | hvu-miR1436 |
miRBase 2 : MIMAT0030070 |
-
Chromosome: 4
-
Position: 1,212,018-1,212,144 (127 nt)
-
Strand: +
-
G+C content: 33.07 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -66.10 kcal/mol |
AMFE -52.05 |
MFEI -1.57
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 94 [download]
- Distribution of reads: random
UAUUGAACAUUGAGUAGGAUCUUAAGUGGUAUAUAGAAUAUGAGCCUCUCCACUCAUUGCCAAUUGAUUUUGAGUGGAGAUGCUUAUAUCUUAUAUAUAUCACUUAGAUCCUACUCAAGUUUCAAUG
(((((((..(((((((((((((.((((((((((((..((((((((.((((((((((..............)))))))))).))))))))....)))))))))))))))))))))))))..)))))))
...************************.................................................................................................... length=24 depth=5
.........******************.................................................................................................... length=18 depth=2
............***********************............................................................................................ length=23 depth=2
..............*********************............................................................................................ length=21 depth=2
.....................************************.................................................................................. length=24 depth=3
.......................***********************................................................................................. length=23 depth=2
.......................************************................................................................................ length=24 depth=41
........................***********************................................................................................ length=23 depth=3
.........................**********************................................................................................ length=22 depth=2
..............................************************......................................................................... length=24 depth=7
............................................................................................************************........... length=24 depth=4
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,230,915-1,231,313 (399 nt)
-
Strand: -
-
G+C content: 33.58 %
- miRNA sequence: AUCACAAUCGCGGUACAGUAAACG (24 nt)
- miRNA depth: 7 (weight: 7)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
[alternative sequences]
-
Stability of the secondary structure of the precursor: MFE -70.02 kcal/mol |
AMFE -33.20 |
MFEI -0.99
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 36 [download]
- Distribution of reads: one island ★
(((((((((((((.((((((.((((((((((((((((((((((((((.(..........((((((((...((((((((((((((....)).))).)))))))))))))))))..((.((((((((.(((((((....((((.((((((((.((..((((((...))))))..))..))))))))))))......)))))))..))))).))).))(((((.....((((.......))))(((((((...((..((((((((......................................)))))))).))...))))))).))))).(((((....)))))........).)))))))))))))))))))))))))).)))))).)))))))))))))
AUCCGCGUUUAUUAUACCGCUAUUGUGAUUUACCCGACUAAUCCCACUCACACAAAAAAGCAAUCUAUGUGAUCAAUGAGAGUAGUUCUAGUUCUUUAUUGAUUUAGAUUGUCAUUGGAACAAUCUUCAACCUUUUUUGUCUAUCUUGGAUGAUGUAUUUUCGGAAAAUGAUUCUCUUUAAGAUGAUAUUAUUUAGGUUGACUGAUUGAUUUGGAGUCUUAUCUAUCCUUAUGAUUAGGAGUUUUAUCUAGUUCGAUUUGUCAUUUUCCUUACCAAUUAACACAACACAUAACAUAUAUAGAUAGAUUCACAUUAUAAAACAAAGACAAAACGACGUCGUUUCUUAACGGGUGUGGGAUUAGUUGGGUAAAUCACAAUCGCGGUACAGUAAACGCGGAU
(((((((((((((.((((((.((((((((((((((((((((((((((.(.............................................................................................................................................................................................................................................................................................................).)))))))))))))))))))))))))).)))))).)))))))))))))
[[[[[[[[.[[[[[[.[[[[[[[[ ]]]]]]]].]]]]]].]]]]]]]]
...............************************........................................................................................................................................................................................................................................................................................................................................................................ length=24 depth=2
....................................................................************************................................................................................................................................................................................................................................................................................................................... length=24 depth=3
....................................................................................................************************................................................................................................................................................................................................................................................................................... length=24 depth=3
.............................................................................................................************************.......................................................................................................................................................................................................................................................................... length=24 depth=3
...................................................................................................................................................................................************************.................................................................................................................................................................................................... length=24 depth=2
..................................................................................................................................................................................................................................................................................................................................................................................AUCACAAUCGCGGUACAGUAAACG..... length=24 depth=7
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,231,264-1,231,345 (82 nt)
-
Strand: +
-
G+C content: 40.24 %
- miRNA sequence: AAACGCGGAUUUGUGGUUAAUCUC (24 nt)
- miRNA depth: 48 (weight: 24)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -22.20 kcal/mol |
AMFE -27.07 |
MFEI -0.67
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 69 [download]
- Distribution of reads: one island ★
UGAGUGGGAUUAGUCGGGUAAAUCACAAUAGCGGUAUAAUAAACGCGGAUUUGUGGUUAAUCUCGAUUUAAUGACCGGUUCA
(((((.((((((((((((..((((((((..(((..........)))....))))))))...))))).)))))..)).)))))
........................................***********************................... length=23 depth=4
........................................AAACGCGGAUUUGUGGUUAAUCUC.................. length=24 depth=48
.........................................************************................. length=24 depth=11
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,270,783-1,270,953 (171 nt)
-
Strand: -
-
G+C content: 47.95 %
- miRNA sequence: CGGAGAAGCUGUAGGCGCUUUAUC (24 nt)
- miRNA depth: 13 (weight: 13)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
[optimal MFE structure]
-
Stability of the secondary structure of the precursor: MFE -23.20 kcal/mol |
AMFE -29.53 |
MFEI -0.62
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 66 [download]
- Distribution of reads: one island ★
(((((((((..(.(((((((...((((((((((.....(((((((.(((....)))...)))))))((((((..((..(((..((((....))))..))))).))))))((((((.......))))))......))))))))))....))))))))..))....)))))))
GCGGAGAAGCUGUAGGCGCUUUAUCGAGAGGUUUUGUGUACGGGCUUCUGCUAAGGUUUGCUCGUGGCAACUAGGAUGGGACUCCAACUCUUUGGUAUCUUCGAGUUGUGGAAAUCACGCAAAUUUCCGAGUUUAGCUUCUCGGAAUCGGUGUUUCUUCUCUUAUUUCCGC
(((((((((..(.(((((((...((((((((((.....................................................................................................))))))))))....))))))))..))....)))))))
[[[[[[[[..[.[[[[[[[...[[ ]]....]]]]]]]]..]]....]]]]]]
***********************.................................................................................................................................................... length=23 depth=2
.CGGAGAAGCUGUAGGCGCUUUAUC.................................................................................................................................................. length=24 depth=13
......************************............................................................................................................................................. length=24 depth=3
.............................................................************************...................................................................................... length=24 depth=2
..........................................................................************************......................................................................... length=24 depth=3
...........................................................................************************........................................................................ length=24 depth=2
............................................................................................************************....................................................... length=24 depth=2
...................................................................................................**********************.................................................. length=22 depth=2
.................................................................................................................................************************.................. length=24 depth=2
..................................................................................................................................************************................. length=24 depth=3
...................................................................................................................................************************................ length=24 depth=2
....................................................................................................................................************************............... length=24 depth=3
.....................................................................................................................................************************.............. length=24 depth=2
.........................................................................................................................................************************.......... length=24 depth=2
.................................................................................................................................................************************.. length=24 depth=2
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,438,780-1,438,882 (103 nt)
-
Strand: +
-
G+C content: 35.92 %
- miRNA sequence: UGUCCGUUGGAGAUUCUAUUGCCU (24 nt)
- miRNA depth: 7 (weight: 7)
- Other precursors with the same miRNA: none
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -32.90 kcal/mol |
AMFE -31.94 |
MFEI -0.89
- Stability of the miRNA duplex (mirdup): yes ★
-
Total number of reads mapped to the precursor: 17 [download]
- Distribution of reads: random
CAUUUUCUGGGCAAAUCAAAGAAAUUGGAAUCUUUAAUAGACUAAACAUUGAUGUCAAGGAUAUGAGUGAUUGUCCGUUGGAGAUUCUAUUGCCUUGGUGAUG
((((.((.((((((...........(((((((((((((.(((.((.((((.(((((...))))).)))).))))).))))))))))))))))))).)).))))
[[[[[[...........[[[[[[[[[[[[[.[[[.[ ]]]].]]]]]]]]]]]]]]]]]]]
........................................***********************........................................ length=23 depth=2
...........................................************************.................................... length=24 depth=2
..............................................................************************................. length=24 depth=2
.......................................................................UGUCCGUUGGAGAUUCUAUUGCCU........ length=24 depth=7
miRBase alignment: none
-
Chromosome: 4
-
Position: 1,439,203-1,439,307 (105 nt)
-
Strand: -
-
G+C content: 28.57 %
-
miRNA precursor: [FASTA sequence]
[stem-loop structure]
-
Stability of the secondary structure of the precursor: MFE -24.30 kcal/mol |
AMFE -23.14 |
MFEI -0.81
- Stability of the miRNA duplex (mirdup): no
-
Total number of reads mapped to the precursor: 98 [download]
- Distribution of reads: random
CCAAUAGUGAGAAGAGAAAUGUCAUGAUGGGUUGUAAAGAGUAUCUCAGAAUUAACUUUCUACACUUUAUUUCCGUCAUUUUCAUUAUUUUUUUUUUUUAUUGUG
(((((((.(((((((((((((..((((((((..((((((.((.....((((......)))))).)))))).))))))))...)))).))))))))).)))))).)
..************************............................................................................... length=24 depth=5
.....************************............................................................................ length=24 depth=17
.........************************........................................................................ length=24 depth=3
..........************************....................................................................... length=24 depth=2
...........*********************......................................................................... length=21 depth=2
...........***********************....................................................................... length=23 depth=3
...........************************...................................................................... length=24 depth=8
............************************..................................................................... length=24 depth=10
.............***********************..................................................................... length=23 depth=13
.............************************.................................................................... length=24 depth=3
.................************************................................................................ length=24 depth=2
..................************************............................................................... length=24 depth=6
.....................************************............................................................ length=24 depth=3
.......................************************.......................................................... length=24 depth=2
.........................***********************......................................................... length=23 depth=7
miRBase alignment: none