An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor - Archive ouverte HAL
Journal Articles Bioresource Technology Year : 2016

An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor

Abstract

Culture medium elements were analysed by a screening DoE to identify their influence in surfactin specific production by a surfactin constitutive overproducing Bacillus subtilis strain. Statistics pointed the major enhancement caused by high glutamic acid concentrations, as well as a minor positive influence of tryptophan and glucose. Successively, a central composite design was performed in microplate bioreactors using a BioLector((R)), in which variations of these impressive parameters, glucose, glutamic acid and tryptophan concentrations were selected for optimization of product-biomass yield (Y-P/X). Results were exploited in combination with a RSM. In absolute terms, experiments attained an Y-P/X 3.28-fold higher than those obtained in Landy medium, a usual culture medium used for lipopeptide production by B. subtilis. Therefore, two medium compositions for enhancing biomass and surfactin specific production were proposed and tested in continuous regime in a bubbleless membrane bioreactor. An Y-P/X increase of 2.26-fold was observed in bioreactor scale.
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hal-04431708 , version 1 (01-02-2024)

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Luiz Fernando Motta dos Santos, François Coutte, Rozenn Ravallec, Pascal Dhulster, Lucie Tournier-Couturier, et al.. An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor. Bioresource Technology, 2016, 218, pp.944-952. ⟨10.1016/j.biortech.2016.07.053⟩. ⟨hal-04431708⟩
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