Assessment of Lipopeptide Mixtures Produced by Bacillus subtilis as Biocontrol Products against Apple Scab (Venturia inaequalis) - Archive ouverte HAL
Article Dans Une Revue Microorganisms Année : 2022

Assessment of Lipopeptide Mixtures Produced by Bacillus subtilis as Biocontrol Products against Apple Scab (Venturia inaequalis)

Aline Leconte
  • Fonction : Auteur
Ludovic Tournant
  • Fonction : Auteur
Jérôme Muchembled
Jonathan Paucellier
  • Fonction : Auteur
Arnaud Héquet
  • Fonction : Auteur
Caroline Deweer
Magali Deleu
Sandrine Oste
  • Fonction : Auteur
Philippe Jacques

Résumé

Apple scab is an important disease conventionally controlled by chemical fungicides, which should be replaced by more environmentally friendly alternatives. One of these alternatives could be the use of lipopeptides produced by Bacillus subtilis. The objective of this work is to study the action of the three families of lipopeptides and different mixtures of them in vitro and in vivo against Venturia inaequalis. Firstly, the antifungal activity of mycosubtilin/surfactin and fengycin/surfactin mixtures was determined in vitro by measuring the median inhibitory concentration. Then, the best lipopeptide mixture ratio was produced using Design of Experiment (DoE) to optimize the composition of the culture medium. Finally, the lipopeptides mixtures efficiency against V. inaequalis was assessed in orchards as well as the evaluation of the persistence of lipopeptides on apple. In vitro tests show that the use of fengycin or mycosubtilin alone is as effective as a mixture, with the 50–50% fengycin/surfactin mixture being the most effective. Optimization of culture medium for the production of fengycin/surfactin mixture shows that the best composition is glycerol coupled with glutamic acid. Finally, lipopeptides showed in vivo antifungal efficiency against V. inaequalis regardless of the mixture used with a 70% reduction in the incidence of scab for both mixtures (fengycin/surfactin or mycosubtilin/surfactin). The reproducibility of the results over the two trial campaigns was significantly better with the mycosubtilin/surfactin mixture. The use of B. subtilis lipopeptides to control this disease is very promising.
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Dates et versions

hal-04431692 , version 1 (20-04-2024)

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Citer

Aline Leconte, Ludovic Tournant, Jérôme Muchembled, Jonathan Paucellier, Arnaud Héquet, et al.. Assessment of Lipopeptide Mixtures Produced by Bacillus subtilis as Biocontrol Products against Apple Scab (Venturia inaequalis). Microorganisms, 2022, 10 (9), pp.1810. ⟨10.3390/microorganisms10091810⟩. ⟨hal-04431692⟩
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