Optical Methods and Their Limitation to Characterize the Morphology and Granulometry of Complex Shape Biological Materials
Résumé
Particle size and morphology of biomass (microorganism, lignocellulosic substrates) stand out as the major determinants of the bioprocess efficiency. Through its impact on rheology, it affects momentum, heat and and mass transfers within the bioreactor. Various techniques are available to characterize in-situ and ex-situ size and shape of particles. The most common methods are classified into three groups: (i) analysis of microscopic images; (ii) laser light diffraction and (iii) settling kinetics. In present work, five techniques are compared and discussed with model particles, microorganisms and lignocellulosic substrates.
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