Root associated Streptomyces produce galbonolides to modulate plant immunity and promote rhizosphere colonisation - Archive ouverte HAL Access content directly
Preprints, Working Papers, ... Year : 2024

Root associated Streptomyces produce galbonolides to modulate plant immunity and promote rhizosphere colonisation

Abstract

The rhizosphere, which serves as the primary interface between plant roots and the soil, constitutes an ecological niche for a huge diversity of microbial communities. Currently, there is little knowledge on the nature and the function of the different metabolites released by rhizospheric microbes to facilitate colonization of this highly competitive environment. Here, we demonstrate how the production of galbonolides, a group of polyene macrolides that inhibit plant and fungal Inositol Phosphorylceramide Synthase (IPCS), empowers the rhizospheric Streptomyces strain AgN23, to thrive in the rhizosphere by triggering the plant-defence mechanisms. Metabolomic analysis of AgN23-inoculated Arabidopsis roots revealed a strong induction in the production of an indole alkaloid, camalexin, which is a major phytoalexin in Arabidopsis. By using a plant mutant compromised in camalexin synthesis, we show that camalexin production is necessary for the successful colonization of the rhizosphere by AgN23. Conversely, hindering galbonolides biosynthesis in AgN23 knock-out mutant resulted in loss of inhibition of IPCS, a deficiency in plant defence activation, notably the production of camalexin, and a strongly reduced development of the mutant bacteria in the rhizosphere. Together, our results throw light on the mechanism by which streptomycetes induce the production of a root defence compound to support their colonisation of the rhizosphere.
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hal-04412369 , version 1 (23-01-2024)

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Clement Nicolle, Damien Gayrard, Alba Noel, Marion Hortala, Aurelien Amiel, et al.. Root associated Streptomyces produce galbonolides to modulate plant immunity and promote rhizosphere colonisation. 2024. ⟨hal-04412369⟩
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