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Article Dans Une Revue Infection, Genetics and Evolution Année : 2017

Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence

Consuelo Rubio-Guerri
  • Fonction : Auteur
Juliette Hayer
Daniel García-Párraga
  • Fonction : Auteur
Elvira Nieto-Pelegrín
  • Fonction : Auteur
Mar Melero
  • Fonction : Auteur
Teresa Álvaro
  • Fonction : Auteur
  • PersonId : 1339373
Mónica Valls
  • Fonction : Auteur
  • PersonId : 1339374
Jose Manuel Sánchez-Vizcaíno
  • Fonction : Auteur
Sándor Belák
  • Fonction : Auteur
  • PersonId : 1339375
Fredrik Granberg
  • Fonction : Auteur
  • PersonId : 1339376

Résumé

Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population (Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080 bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of this genome is a remarkably long 4380 bp E3 ORF1, that displays no sequence homology with the corresponding E3 regions of other adenoviruses. In addition, the fiber protein only has 26% identity with fiber proteins described in other adenoviruses. Three hypothetical proteins were predicted. The phylogenetic analysis indicates that the closest known relative to BdAdV-1 is an adenovirus detected in bottlenose dolphin (KR024710), with an amino acid sequence identity between 36 and 79% depending on the protein. Based on the phylogenic analysis, the BdAdV-1 appears to have co-evolved with its host. The results indicate that BdAdV-1 belongs to the Mastadenovirus genus of the Adenoviridae family, however, it is clearly different from other adenoviruses, especially in the 3′-end of the viral genome. The high degree of sequence divergence suggests that BdAdV-1 should be considered as a novel species in the Mastadenovirus genus. The study also demonstrates the usefulness of high-throughput sequencing to obtain full-length genomes of genetically divergent viruses.
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Origine : Publication financée par une institution
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-04409148 , version 1 (22-01-2024)

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Maja Malmberg, Consuelo Rubio-Guerri, Juliette Hayer, Daniel García-Párraga, Elvira Nieto-Pelegrín, et al.. Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence. Infection, Genetics and Evolution, 2017, 53, pp.47 - 55. ⟨10.1016/j.meegid.2017.05.008⟩. ⟨hal-04409148⟩
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