Glycosaminoglycan-mimetic infernan grafted with poly(N-isopropylacrylamide): Toward a thermosensitive polysaccharide - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Carbohydrate Polymers Année : 2024

Glycosaminoglycan-mimetic infernan grafted with poly(N-isopropylacrylamide): Toward a thermosensitive polysaccharide

Résumé

Glycosaminoglycans (GAGs) are essential constituents of the cell surface and extracellular matrix, where they are involved in several cellular processes through their interactions with various proteins. For successful tissue regeneration, developing an appropriate matrix supporting biological activities of cells in a similar manner than GAGs remains still challenging. In this context, this study aims to design a thermosensitive polysaccharide that could further be used as hydrogel for tissue engineering applications. For this purpose, infernan, a marine bacterial exopolysaccharide (EPS) endowed with GAG-mimetic properties was grafted with a thermosensitive polymer, poly(N-isopropylacrylamide) (pNIPAM). Eight grafted polysaccharides were obtained by varying EPS/ pNIPAM molar ratio and the molecular weight of pNIPAM. Their physicochemical characteristics and their thermosensitive properties were determined using a multi-technique, experimental approach. In parallel, molecular dynamics and Monte Carlo simulations were applied at two different scales to elucidate, respectively, the molecular conformation of grafted infernan chain and their ability to form an infinite network undergoing a solgel transition near the percolation, a necessary condition in hydrogel formation. It comes out from this study that thermosensitive infernan was successfully developed and its potential use in tissue regeneration as a hydrogel scaffold will further be assessed.
Fichier sous embargo
Fichier sous embargo
0 1 0
Année Mois Jours
Avant la publication
mardi 28 mai 2024
Fichier sous embargo
mardi 28 mai 2024
Connectez-vous pour demander l'accès au fichier

Dates et versions

hal-04406555 , version 1 (26-04-2024)

Identifiants

Citer

Arnaud Fillaudeau, Stéphane Cuenot, Olga Makshakova, Serena Traboni, Corinne Sinquin, et al.. Glycosaminoglycan-mimetic infernan grafted with poly(N-isopropylacrylamide): Toward a thermosensitive polysaccharide. Carbohydrate Polymers, 2024, 326, pp.121638. ⟨10.1016/j.carbpol.2023.121638⟩. ⟨hal-04406555⟩
6 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More