Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Science Advances Année : 2024

Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems

David Bikard

Résumé

Integrons are adaptive bacterial devices that rearrange promoter-less gene cassettes into variable ordered arrays under stress conditions, thereby sampling combinatorial phenotypic diversity. Chromosomal integrons often carry hundreds of silent gene cassettes, with integrase-mediated recombination leading to rampant DNA excision and integration, posing a potential threat to genome integrity. How this activity is regulated and controlled, particularly through selective pressures, to maintain such large cassette arrays is unknown. Here, we show a key role of promoter-containing toxin-antitoxin (TA) cassettes as systems that kill the cell when the overall cassette excision rate is too high. These results highlight the importance of TA cassettes regulating the cassette recombination dynamics and provide insight into the evolution and success of integrons in bacterial genomes.
Fichier principal
Vignette du fichier
MFP_Sci. Adv_2024_Richard.pdf (4.08 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

hal-04397951 , version 1 (16-01-2024)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Egill Richard, Baptiste Darracq, Eloi Littner, Claire Vit, Clémence Whiteway, et al.. Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems. Science Advances , 2024, 10 (2), pp.eadj3498. ⟨10.1126/sciadv.adj3498⟩. ⟨hal-04397951⟩
27 Consultations
11 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More