Isolating plasma extracellular vesicles from mouse blood using size-exclusion chromatography, density gradient, and ultracentrifugation - Archive ouverte HAL
Article Dans Une Revue STAR Protocols Année : 2023

Isolating plasma extracellular vesicles from mouse blood using size-exclusion chromatography, density gradient, and ultracentrifugation

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Circulating extracellular vesicles (EVs) could serve for the surveillance of diverse pathological conditions. We present a protocol for enriching and isolating plasma EVs from mouse blood. We describe steps for employing ultracentrifugation, size-exclusion chromatography, and density gradients, required for further quantitative and qualitative analysis. We detail the procedure for retrieving optimal volume of blood while preserving its integrity and avoiding hemolysis. We also describe the preparation of EVs from this complex fluid containing soluble proteins, aggregates, and lipoprotein particles.
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hal-04391386 , version 1 (10-10-2024)

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Gwennan André-Grégoire, Quentin Roux, Julie Gavard. Isolating plasma extracellular vesicles from mouse blood using size-exclusion chromatography, density gradient, and ultracentrifugation. STAR Protocols, 2023, 4 (4), pp.102740. ⟨10.1016/j.xpro.2023.102740⟩. ⟨hal-04391386⟩
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