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Article Dans Une Revue Developmental Cell Année : 2024

Birth, cell fate and behavior of progenitors at the origin of the cardiac mitral valve

Résumé

Congenital heart malformations include mitral valve defects, which remain largely unexplained. During embryogenesis, a restricted population of endocardial cells within the atrioventricular canal undergoes an endothelial-to-mesenchymal transition to give rise to mitral valvular cells. However, the identity and fate decisions of these progenitors as well as the behavior and distribution of their derivatives in valve leaflets remain unknown. We used single-cell RNA sequencing (scRNA-seq) of genetically labeled endocardial cells and microdissected mouse embryonic and postnatal mitral valves to characterize the developmental road. We defined the metabolic processes underlying the specification of the progenitors and their contributions to subtypes of valvular cells. Using retrospective multicolor clonal analysis, we describe specific modes of growth and behavior of endocardial cell-derived clones, which build up, in a proper manner, functional valve leaflets. Our data identify how both genetic and metabolic mechanisms specifically drive the fate of a subset of endocardial cells toward their distinct clonal contribution to the formation of the valve.
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Dates et versions

hal-04388535 , version 1 (11-01-2024)

Licence

Paternité - Pas d'utilisation commerciale

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Batoul Farhat, Ignacio Bordeu, Bernd Jagla, Stéphanie Ibrahim, Sonia Stefanovic, et al.. Birth, cell fate and behavior of progenitors at the origin of the cardiac mitral valve. Developmental Cell, 2024, ⟨10.1016/j.devcel.2023.12.006⟩. ⟨hal-04388535⟩
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