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Article Dans Une Revue Journal of Cell Science Année : 2023

Targeted volume correlative light and electron microscopy of an environmental marine microorganism

Karel Mocaer
  • Fonction : Auteur
Giulia Mizzon
  • Fonction : Auteur
Manuel Gunkel
  • Fonction : Auteur
Aliaksandr Halavatyi
Anna Steyer
Viola Oorschot
Martin Schorb
Daniel Yee
  • Fonction : Auteur
Fabien Chevalier
  • Fonction : Auteur
Benoit Gallet
Johan Decelle
  • Fonction : Auteur
Yannick Schwab
Paolo Ronchi

Résumé

ABSTRACT Photosynthetic microalgae are responsible for an important fraction of CO2 fixation and O2 production on Earth. Three-dimensional (3D) ultrastructural characterization of these organisms in their natural environment can contribute to a deeper understanding of their cell biology. However, the low throughput of volume electron microscopy (vEM) methods along with the complexity and heterogeneity of environmental samples pose great technical challenges. In the present study, we used a workflow based on a specific electron microscopy sample preparation method compatible with both light and vEM imaging in order to target one cell among a complex natural community. This method revealed the 3D subcellular landscape of a photosynthetic dinoflagellate, which we identified as Ensiculifera tyrrhenica, with quantitative characterization of multiple organelles. We show that this cell contains a single convoluted chloroplast and show the arrangement of the flagellar apparatus with its associated photosensitive elements. Moreover, we observed partial chromatin unfolding, potentially associated with transcription activity in these organisms, in which chromosomes are permanently condensed. Together with providing insights in dinoflagellate biology, this proof-of-principle study illustrates an efficient tool for the targeted ultrastructural analysis of environmental microorganisms in heterogeneous mixes.

Dates et versions

hal-04384387 , version 1 (10-01-2024)

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Citer

Karel Mocaer, Giulia Mizzon, Manuel Gunkel, Aliaksandr Halavatyi, Anna Steyer, et al.. Targeted volume correlative light and electron microscopy of an environmental marine microorganism. Journal of Cell Science, 2023, 136 (15), ⟨10.1242/jcs.261355⟩. ⟨hal-04384387⟩
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