Neutrophil Activation and Immune Thrombosis Profiles Persist in Convalescent COVID-19
Hakim Hocini
(1)
,
Aurélie Wiedemann
(1)
,
Fabiola Blengio
(1)
,
Cécile Lefebvre
(1)
,
Minerva Cervantes-Gonzalez
(2, 3)
,
Emile Foucat
(1)
,
Pascaline Tisserand
(1)
,
Mathieu Surenaud
(1)
,
Séverin Coléon
(1)
,
Mélanie Prague
(4, 5, 6)
,
Lydia Guillaumat
(1)
,
Corinne Krief
(1)
,
Craig Fenwick
(7)
,
Cédric Laouénan
(2, 3)
,
Lila Bouadma
(3)
,
Jade Ghosn
(3, 8)
,
Giuseppe Pantaleo
(1, 7)
,
Rodolphe Thiébaut
(4, 5)
,
Laurent Abel
(9)
,
Amal Abrous
(9)
,
Claire Andrejak
(9)
,
François Angoulvant
(9)
,
Delphine Bachelet
(9)
,
Marie Bartoli
(9)
,
Sylvie Behilill
(9)
,
Marine Beluze
(9)
,
Krishna Bhavsar
(9)
,
Anissa Chair
(9)
,
Charlotte Charpentier
(9)
,
Léo Chenard
(9)
,
Catherine Chirouze
(9)
,
Sandrine Couffin-Cadiergues
(9)
,
Camille Couffignal
(9)
,
Nathalie De Castro
(9)
,
Marie-Pierre Debray
(9)
,
Dominique Deplanque
(9, 10)
,
Diane Descamps
(9)
,
Alpha Diallo
(9)
,
Fernanda Dias Da Silva
(9)
,
Céline Dorival
(9)
,
Xavier Duval
(9)
,
Philippine Eloy
(9)
,
Vincent Enouf
(9)
,
Hélène Esperou
(9)
,
Marina Esposito-Farese
(9)
,
Manuel Etienne
(9)
,
Aline-Marie Florence
(9)
,
Alexandre Gaymard
(9)
,
Tristan Gigante
(9)
,
Morgane Gilg
(9)
,
François Goehringer
(9)
,
Jérémie Guedj
(9)
,
Ikram Houas
(9)
,
Isabelle Hoffmann
(9)
,
Jean-Sébastien Hulot
(9, 11)
,
Salma Jaafoura
(9)
,
Simon Jamard
(9)
,
Ouifiya Kafif
(9)
,
Antoine Khalil
(9)
,
Nadhem Lafhej
(9)
,
Samira Laribi
(9)
,
Minh Le
(9)
,
Quentin Le Hingrat
(9)
,
Soizic Le Mestre
(9)
,
Sophie Letrou
(9)
,
Bruno Lina
(9)
,
Guillaume Lingas
(9)
,
Denis Malvy
(9)
,
France Mentré
(9)
,
Hugo Mouquet
(9)
,
Nadège Neant
(9)
,
Christelle Paul
(9)
,
Aurélie Papadopoulos
(9)
,
Ventzislava Petrov-Sanchez
(9)
,
Gilles Peytavin
(9)
,
Valentine Piquard
(9)
,
Olivier Picone
(9)
,
Manuel Rosa-Calatrava
(9)
,
Bénédicte Rossignol
(9)
,
Patrick Rossignol
(9)
,
Carine Roy
(9)
,
Marion Schneider
(9)
,
Coralie Tardivon
(9)
,
Jean-François Timsit
(9)
,
Sarah Tubiana
(9)
,
Sylvie Van. Der Werf
(9)
,
Benoit Visseaux
(9)
,
Yves Lévy
(12)
1
U955 Inserm - UPEC -
IMRB - "From pathophysiology towards immune-basedinterventions in HIV infection" [Créteil]
2 CIC 1425 - Centre d'investigation Clinique [CHU Bichat] - Épidémiologie clinique
3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution
4 SISTM - Statistics In System biology and Translational Medicine
5 BPH - Bordeaux population health
6 VRI - Vaccine Research Institute [Créteil, France]
7 CHUV - Centre Hospitalier Universitaire Vaudois = Lausanne University Hospital [Lausanne]
8 Services de Maladies Infectieuses et Tropicales [CHU Bichat]
9 French COVID cohort study group
10 LilNCog - Lille Neurosciences & Cognition - U 1172
11 CIC 1418 - CIC - HEGP
12 Groupe Henri Mondor-Albert Chenevier
2 CIC 1425 - Centre d'investigation Clinique [CHU Bichat] - Épidémiologie clinique
3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution
4 SISTM - Statistics In System biology and Translational Medicine
5 BPH - Bordeaux population health
6 VRI - Vaccine Research Institute [Créteil, France]
7 CHUV - Centre Hospitalier Universitaire Vaudois = Lausanne University Hospital [Lausanne]
8 Services de Maladies Infectieuses et Tropicales [CHU Bichat]
9 French COVID cohort study group
10 LilNCog - Lille Neurosciences & Cognition - U 1172
11 CIC 1418 - CIC - HEGP
12 Groupe Henri Mondor-Albert Chenevier
Mélanie Prague
- Fonction : Auteur
- PersonId : 11146
- IdHAL : mprague
- ORCID : 0000-0001-9809-7848
- IdRef : 175063427
Rodolphe Thiébaut
- Fonction : Auteur
- PersonId : 175125
- IdHAL : rodolphe-thiebaut
- ORCID : 0000-0002-5235-3962
- IdRef : 071580735
Claire Andrejak
- Fonction : Auteur
- PersonId : 1144520
- IdHAL : claire-andrejak
- ORCID : 0000-0002-9998-8578
- IdRef : 11107410X
Catherine Chirouze
- Fonction : Auteur
- PersonId : 760630
- ORCID : 0000-0002-6150-2376
- IdRef : 058486186
Alexandre Gaymard
- Fonction : Auteur
- PersonId : 1259371
- ORCID : 0000-0002-8194-6126
Bruno Lina
- Fonction : Auteur
- PersonId : 837368
- ORCID : 0000-0002-8959-2123
- IdRef : 075685132
Manuel Rosa-Calatrava
- Fonction : Auteur
- PersonId : 919324
- ORCID : 0000-0002-7559-9527
Résumé
Purpose Following a severe COVID-19 infection, a proportion of individuals develop prolonged symptoms. We investigated the immunological dysfunction that underlies the persistence of symptoms months after the resolution of acute COVID-19. Methods We analyzed cytokines, cell phenotypes, SARS-CoV-2 spike-specific and neutralizing antibodies, and whole blood gene expression profiles in convalescent severe COVID-19 patients 1, 3, and 6 months following hospital discharge. Results We observed persistent abnormalities until month 6 marked by (i) high serum levels of monocyte/macrophage and endothelial activation markers, chemotaxis, and hematopoietic cytokines; (ii) a high frequency of central memory CD4 + and effector CD8 + T cells; (iii) a decrease in anti-SARS-CoV-2 spike and neutralizing antibodies; and (iv) an upregulation of genes related to platelet, neutrophil activation, erythrocytes, myeloid cell differentiation, and RUNX1 signaling. We identified a “core gene signature” associated with a history of thrombotic events, with upregulation of a set of genes involved in neutrophil activation, platelet, hematopoiesis, and blood coagulation. Conclusion The lack of restoration of gene expression to a normal profile after up to 6 months of follow-up, even in asymptomatic patients who experienced severe COVID-19, signals the need to carefully extend their clinical follow-up and propose preventive measures.
Origine | Fichiers produits par l'(les) auteur(s) |
---|