Color TSFG microscopy of red blood cells and oxygenation
Résumé
We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.