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Article Dans Une Revue Viruses Année : 2023

An Oil-Based Adjuvant Improves Immune Responses Induced by Canine Adenovirus-Vectored Vaccine in Mice

Manon Broutin
Fleur Costa
  • Fonction : Auteur
Sandy Peltier
  • Fonction : Auteur
Jennifer Maye
  • Fonction : Auteur
Nicolas Versillé
  • Fonction : Auteur

Résumé

There is a significant need for highly effective vaccines against emerging and common veterinary infectious diseases. Canine adenovirus type 2 (CAV2) vectors allow rapid development of multiple vaccines and have demonstrated their potential in animal models. In this study, we compared the immunogenicity of a non-replicating CAV2 vector encoding the rabies virus glycoprotein with and without MontanideTM ISA 201 VG, an oil-based adjuvant. All vaccinated mice rapidly achieved rabies seroconversion, which was associated with complete vaccine protection. The adjuvant increased rabies antibody titers without any significant effect on the anti-CAV2 serological responses. An RT2 Profiler™ PCR array was conducted to identify host antiviral genes modulated in the blood samples 24 h after vaccination. Functional analysis of differentially expressed genes revealed the up-regulation of the RIG-I, TLRs, NLRs, and IFNs signaling pathways. These results demonstrate that a water-in-oil-in-water adjuvant can shape the immune responses to an antigen encoded by an adenovirus, thereby enhancing the protection conferred by live recombinant vaccines. The characterization of early vaccine responses provides a better understanding of the mechanisms underlying the efficacy of CAV2-vectored vaccines.

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hal-04369775 , version 1 (02-01-2024)

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Manon Broutin, Fleur Costa, Sandy Peltier, Jennifer Maye, Nicolas Versillé, et al.. An Oil-Based Adjuvant Improves Immune Responses Induced by Canine Adenovirus-Vectored Vaccine in Mice. Viruses, 2023, 15 (8), pp.1664. ⟨10.3390/v15081664⟩. ⟨hal-04369775⟩
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