Soil pollution, a difficult environmental problem, has been attracting considerable public attention over the last decades. Several organic persistent pollutants are found in contaminated soils including dioxins/furans (PCDD/F). These contaminants are harmful both for environment and human health (immunotoxicity, neurotoxicity, carcinogenicity). PCDD/F are characterized by high hydrophobicity and recalcitrance to oxidation, which make them highly resistant to degradation. In the current work, Arbuscular Mycorrhizal Fungi (AMF) –assisted phytormediation is proposed to restore aged PCDD/F polluted soil. This technique is environmental friendly, potentially cost effective and not altering the soil matrix. Moreover, AMF improves the phytotechnology effectiveness by providing protection to the plants against the pollutant toxicity and by enhancing pollutant dissipation. The contribution of mycorrhizal inoculum addition on the ability of four plant species (Clover, Alfalfa, Ryegrass and Tall fescue) to grow and to dissipate PCDD/F was assessed after 20 weeks of microcosm culture. Our findings showed that the four plant species were able to grow on the soil despite the presence of PCDD/F at the concentration of 200ng/kg. Total and arbusculararbsucular mycorrhizal colonizations of 70 and 30% respectively, were obtained after 20 weeks of culture. PCDD/F residual concentrations in the soil were reduced in the presence of the vegetation. A dissipation of 20% was obtained with Tall fescue. This dissipation was found to be due to the stimulation of the microflora measured through the enzyme activities (FDA hydrolases and dehydrogenases) and bacterial flora, evidenced by bacterial Phospholipid Fatty Acids (PLFA). On the other hand, the evolution of structure and diversity of microbiomes refers to the whole community of bacteria, archaea and fungi after phytoremediation of sterilized or non-sterilized soil. It was showed that bacterial and fungal community structures were significantly affected by AMF inoculation, vegetation and on initial microbial system state.