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Communication Dans Un Congrès Année : 2022

Metagenomic expansion and active site analysis of the Amine Dehydrogenase family

Pelletier Eric

Résumé

The current boom in environmental genomics data provides a huge resource of new sequences of potential biocatalysts. Through the MODAMDH project, we focus on one of the key biocatalysts named amine dehydrogenases (AmDHs) which enable the access to amines that are important entities in the chemical industry [1]. We started from the previously described NAD(P)H-dependent AmDH family from which several members were experimentally characterized [2,3]. This family was first expanded, up to 27k sequences, by mining very large metagenomic databases in search of the conserved catalytic domain. We also generated a pool of ~100k candidate families containing more than 20M NAD(P)-binding protein sequences from which we found, using HMM-HMM profile comparison, new distant homologs. We then applied structural modelling and active site classification to define AmDH subfamilies [4]. From the latter, representative enzyme candidates were further characterized through enzymatic assays with the dual purpose of confirming their AmDH activity and finding new features, particularly in terms of substrate spectrum and complementary stereoselectivities. This work is supported by the Agence Nationale de la Recherche (ANR-19-CE07-0007).
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Dates et versions

hal-04360123 , version 1 (21-12-2023)

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  • HAL Id : hal-04360123 , version 1

Citer

Elisée Eddy, Laurine Ducrot, Raphaël Méheust, Mark Stam, Pelletier Eric, et al.. Metagenomic expansion and active site analysis of the Amine Dehydrogenase family. International Congress on Biocatalysis 2022, Aug 2022, Hamburg, Germany. ⟨hal-04360123⟩
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