The bacterial amyloid-like Hfq promotes in vitro DNA alignment - Archive ouverte HAL
Article Dans Une Revue Microorganisms Année : 2019

The bacterial amyloid-like Hfq promotes in vitro DNA alignment

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The Hfq protein is reported to be involved in environmental adaptation and virulence of several bacteria. In Gram-negative bacteria, Hfq mediates the interaction between regulatory noncoding RNAs and their target mRNAs. Besides these RNA-related functions, Hfq is also associated with DNA and is a part of the bacterial chromatin. Its precise role in DNA structuration is, however, unclear and whether Hfq plays a direct role in DNA-related processes such as replication or recombination is controversial. In previous works, we showed that Escherichia coli Hfq, or more precisely its amyloid-like C-terminal region (CTR), induces DNA compaction into a condensed form. In this paper, we evidence a new property for Hfq; precisely we show that its CTR influences double helix structure and base tilting, resulting in a strong local alignment of nucleoprotein Hfq:DNA fibers. The significance of this alignment is discussed in terms of chromatin structuration and possible functional consequences on evolutionary processes and adaptation to environment.
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hal-04353289 , version 1 (07-02-2024)

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Frank Wien, Denis Martinez, Etienne Le Brun, Nykola Jones, Søren Vrønning Hoffmann, et al.. The bacterial amyloid-like Hfq promotes in vitro DNA alignment. Microorganisms, 2019, 7 (12), pp.639. ⟨10.3390/microorganisms7120639⟩. ⟨hal-04353289⟩
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