Interactions and Insertion of Escherichia coli Hfq into Outer Membrane Vesicles as Revealed by Infrared and Orientated Circular Dichroism Spectroscopies - Archive ouverte HAL
Article Dans Une Revue International Journal of Molecular Sciences Année : 2023

Interactions and Insertion of Escherichia coli Hfq into Outer Membrane Vesicles as Revealed by Infrared and Orientated Circular Dichroism Spectroscopies

Florian Turbant
  • Fonction : Auteur
LLB - Matière molle et biophysique
Jehan Waeytens
Université libre de Bruxelles
Anaïs Blache
  • Fonction : Auteur
CEA Cadarache
Emeline Esnouf
  • Fonction : Auteur
LLB - Matière molle et biophysique
Vincent Raussens
  • Fonction : Auteur
Université libre de Bruxelles
Grzegorz Węgrzyn
University of Gdańsk
Wafa Achouak
  • Fonction : Auteur
CEA Cadarache
Frank Wien
Synchrotron SOLEIL
Véronique Arluison
LLB - Matière molle et biophysique

Résumé

The possible carrier role of Outer Membrane Vesicles (OMVs) for small regulatory noncoding RNAs (sRNAs) has recently been demonstrated. Nevertheless, to perform their function, these sRNAs usually need a protein cofactor called Hfq. In this work we show, by using a combination of infrared and circular dichroism spectroscopies, that Hfq, after interacting with the inner membrane, can be translocated into the periplasm, and then be exported in OMVs, with the possibility to be bound to sRNAs. Moreover, we provide evidence that Hfq interacts with and is inserted into OMV membranes, suggesting a role for this protein in the release of sRNA outside the vesicle. These findings provide clues to the mechanism of host–bacteria interactions which may not be defined solely by protein–protein and protein–outer membrane contacts, but also by the exchange of RNAs, and in particular sRNAs.

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Sciences du Vivant [q-bio]

Veronique Arluison  : Connectez-vous pour contacter le contributeur

https://hal.science/hal-04353217

Soumis le : mardi 19 décembre 2023-12:56:09

Dernière modification le : mardi 23 avril 2024-03:23:21

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Dates et versions

hal-04353217 , version 1 (19-12-2023)

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Florian Turbant, Jehan Waeytens, Anaïs Blache, Emeline Esnouf, Vincent Raussens, et al.. Interactions and Insertion of Escherichia coli Hfq into Outer Membrane Vesicles as Revealed by Infrared and Orientated Circular Dichroism Spectroscopies. International Journal of Molecular Sciences, 2023, 24 (14), pp.11424. ⟨10.3390/ijms241411424⟩. ⟨hal-04353217⟩

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