The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecules Année : 2023

The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation

Paulina Aguilera
  • Fonction : Auteur
Camilo Berríos-Pastén
  • Fonction : Auteur
Marcelo Veloso
  • Fonction : Auteur
Matías Gálvez-Silva
  • Fonction : Auteur
Rosalba Lagos
  • Fonction : Auteur
Frank Wien
  • Fonction : Auteur
Andrés Marcoleta
  • Fonction : Auteur

Résumé

Microcin E492 (MccE492) is an antimicrobial peptide and proposed virulence factor produced by some Klebsiella pneumoniae strains, which, under certain conditions, form amyloid fibers, leading to the loss of its antibacterial activity. Although this protein has been characterized as a model functional amyloid, the secondary structure transitions behind its formation, and the possible effect of molecules that inhibit this process, have not been investigated. In this study, we examined the ability of the green tea flavonoid epigallocatechin gallate (EGCG) to interfere with MccE492 amyloid formation. Aggregation kinetics followed by thioflavin T binding were used to monitor amyloid formation in the presence or absence of EGCG. Additionally, synchrotron radiation circular dichroism (SRCD) and transmission electron microscopy (TEM) were used to study the secondary structure, thermal stability, and morphology of microcin E492 fibers. Our results showed that EGCG significantly inhibited the formation of the MccE492 amyloid, resulting in mainly amorphous aggregates and small oligomers. However, these aggregates retained part of the β-sheet SRCD signal and a high resistance to heat denaturation, suggesting that the aggregation process is sequestered or deviated at some stage but not completely prevented. Thus, EGCG is an interesting inhibitor of the amyloid formation of MccE492 and other bacterial amyloids.

Dates et versions

hal-04353191 , version 1 (19-12-2023)

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Citer

Paulina Aguilera, Camilo Berríos-Pastén, Marcelo Veloso, Matías Gálvez-Silva, Florian Turbant, et al.. The Green Tea Polyphenol Epigallocatechin-Gallate (EGCG) Interferes with Microcin E492 Amyloid Formation. Molecules, 2023, 28 (21), ⟨10.3390/molecules28217262⟩. ⟨hal-04353191⟩
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