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Article Dans Une Revue Frontiers in Immunology Année : 2023

Fluorescent non transgenic schistosoma to decipher host-parasite phenotype compatibility

Résumé

Schistosomiasis is considered as a significant public health problem, imposing a deeper understanding of the intricate interplay between parasites and their hosts. Unfortunately, current invasive methodologies employed to study the compatibility and the parasite development impose limitations on exploring diverse strains under various environmental conditions, thereby impeding progress in the field. In this study, we demonstrate the usefulness for the trematode parasite Schistosoma mansoni, leveranging a fluorescence-imagingbased approach that employs fluorescein 5-chloromethylfluorescein diacetate (CMFDA) and 5-chloromethylfluorescein diacetate (CMAC) as organism tracker for intramolluscan studies involving the host snail Biomphalaria glabrata. These probes represent key tools for qualitatively assessing snail infections with unmatched accuracy and precision. By monitoring the fluorescence of parasites within the snail vector, our method exposes an unprecedented glimpse into the host-parasite compatibility landscape. The simplicity and sensitivity of our approach render it an ideal choice for evolutionary studies, as it sheds light on the intricate mechanisms governing host-parasite interactions. Fluorescent probe-based methods play a pivotal role in characterizing factors influencing parasite development and phenotype of compatibility, paving the way for innovative, effective, and sustainable solutions to enhance our understanding host-parasite immunobiological interaction and compatibility.
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Dates et versions

hal-04324556 , version 1 (05-12-2023)

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David Duval, Pierre Poteaux, Benjamin Gourbal, Anne Rognon, Ronaldo de Carvalho Augusto. Fluorescent non transgenic schistosoma to decipher host-parasite phenotype compatibility. Frontiers in Immunology, 2023, 14, ⟨10.3389/fimmu.2023.1293009⟩. ⟨hal-04324556⟩
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