Poster De Conférence Année : 2010

Segregation of the terminal region of the Escherichia coli chromosome

Jean-Christophe Meile
Marie Deghorain
  • Fonction : Auteur
Carine Pages
  • Fonction : Auteur
François Cornet
Mathieu Stouf
  • Fonction : Auteur

Résumé

The FtsK DNA-translocase controls the last steps of chromosome segregation in E. coli. It is associated with the cell division septum and translocates sister chromosomes using the KOPS DNA motifs to orient its activity, thereby controlling both decatenation by TopoIV and chromosome dimer resolution by XerCD/dif recombination. Using XerCD/dif recombination to probe the interaction of chromosome loci with FtsK, we have shown that FtsK activity is restricted to a ~400 kb region around the dif position, the FtsK High Activity Region (FHAR). Measurement of the relative segregation times of different loci tagged with fluorescent proteins by microscopy shows that this region is subjected to an elevated level of post-replicative cohesion between sister-chromosomes. Using pairs of loci tagged with two different fluorescent proteins, we showed that loci of the FHAR segregate in an ordered manner, the dif site being the last point of contact between sister chromosomes. These data suggest that the oriented translocase activity of FtsK controls the ordered segregation of a specific terminal region of the chromosome.

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hal-04324035 , version 1 (05-12-2023)

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  • HAL Id : hal-04324035 , version 1

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Mathieu Stouf, Jean-Christophe Meile, Marie Deghorain, Carine Pages, François Cornet, et al.. Segregation of the terminal region of the Escherichia coli chromosome. Replication/repair and segregation of chromosomes, Jun 2010, Freiburg, Germany. ⟨hal-04324035⟩
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