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Article Dans Une Revue Nature Communications Année : 2023

Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes

Résumé

Bdelloid rotifers are part of the restricted circle of multicellular animals that can withstand a wide range of genotoxic stresses at any stage of their life cycle. In this study, bdelloid rotifer Adineta vaga is used as a model to decipher the molecular basis of their extreme tolerance. Proteomic analysis shows that a specific DNA ligase, different from those usually involved in DNA repair in eukaryotes, is strongly over-represented upon ionizing radiation. A phylogenetic analysis reveals its orthology to prokaryotic DNA ligase E, and its horizontal acquisition by bdelloid rotifers and plausibly other eukaryotes. The fungus Mortierella verticillata, having a single copy of this DNA Ligase E homolog, also exhibits an increased radiation tolerance with an over-expression of this DNA ligase E following X-ray exposure. We also provide evidence that A. vaga ligase E is a major contributor of DNA breaks ligation activity, which is a common step of all important DNA repair pathways. Consistently, its heterologous expression in human cell lines significantly improves their radio-tolerance. Overall, this study highlights the potential of horizontal gene transfers in eukaryotes, and their contribution to the adaptation to extreme conditions.
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Dates et versions

hal-04321606 , version 1 (04-12-2023)

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Emilien Nicolas, Paul Simion, Marc Guérineau, Matthieu Terwagne, Mathilde Colinet, et al.. Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes. Nature Communications, 2023, Nature Communications, 14 (1), pp.7638. ⟨10.1038/s41467-023-43075-8⟩. ⟨hal-04321606⟩
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