The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2023

The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population

Michel Yvon
Diane Ullman
Eric Verdin

Résumé

Bunyaviruses are enveloped negative or ambisense single-stranded RNA viruses with a genome divided into several segments. The canonical view depicts each viral particle packaging one copy of each genomic segment in one polarity named the viral strand. Several opposing observations revealed nonequal ratios of the segments, uneven number of segments per virion, and even packaging of viral complementary strands. Unfortunately, these observations result from studies often addressing other questions, on distinct viral species, and not using accurate quantitative methods. Hence, what RNA segments and strands are packaged as the genome of any bunyavirus remains largely ambiguous. We addressed this issue by first investigating the virion size distribution and RNA content in populations of the tomato spotted wilt virus (TSWV) using microscopy and tomography. These revealed heterogeneity in viral particle volume and amount of RNA content, with a surprising lack of correlation between the two. Then, the ratios of all genomic segments and strands were established using RNA sequencing and qRT-PCR. Within virions, both plus and minus strands (but no mRNA) are packaged for each of the three L, M, and S segments, in reproducible nonequimolar proportions determined by those in total cell extracts. These results show that virions differ in their genomic content but together build up a highly reproducible genetic composition of the viral population. This resembles the genome formula described for multipartite viruses, with which some species of the order Bunyavirales may share some aspects of the way of life, particularly emerging properties at a supravirion scale.
Fichier sous embargo
Fichier sous embargo
0 0 18
Année Mois Jours
Avant la publication
lundi 20 mai 2024
Fichier sous embargo
lundi 20 mai 2024
Connectez-vous pour demander l'accès au fichier

Dates et versions

hal-04313387 , version 1 (29-11-2023)

Identifiants

Citer

Michel Yvon, Thomas German, Diane Ullman, Ranjit Dasgupta, Maxwell Parker, et al.. The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population. Proceedings of the National Academy of Sciences of the United States of America, 2023, 120 (48), pp.e2309412120. ⟨10.1073/pnas.2309412120⟩. ⟨hal-04313387⟩
34 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More